Content Analysis of Shikimic Acid in the Masson Pine Needles and Antiplatelet-aggregating Activity

Xiao-yi Chen, Li Yuan;Lian Wei;Min Wang;Yi-xiong Lei;

Abstract


Shikimic acid content was measured by high-performance liquid chromatography (HPLC) in the Masson pine needles as one potential source of shikimic acid. The antiplatelet-aggregating activity of shikimic acid was characterized by turbidimetry with an animal model. The shikimic acid level was compared with Oriental arborvitae leafytwigs and Star anise. The results showed that the shikimic acid content in Masson pine needles, Oriental arborvitae leafytwigs and Star anise was 5.71%, 1.74% and 8.95%, respectively. The yield of shikimic acid was the highest when the powder of Masson pine needles was ultrasonically-treated for 60 min in 3.5 mmol/L phosphoric acid. Shikimic acid, when separated by HPLC, exhibited a dose-dependent inhibitory effect on platelet aggregation induced by adenosine diphosphate and collagen in rabbits. Because of the relative high content and good antiplatelet-aggregating activity of shikimic acid, the Masson pine needles can be used as a potential source of shikimic acid.

Keywords


Masson Pine Needles; Shikimic Acid; HPLC; Antiplatelet-aggregating Activity.

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