The Optimization of Multi-strain Fermentation Conditions for a High-HX Esterifying Broth and Its Application in CSFL Production

Zhengkai Xue, Yuan Xue, Cheng Ling


In order to increase the content of ethyl caproate (EC),which is the most important flavor component for the typical features of Chinese Strong-flavor Liquor (CSFL), the mixed -fermentation conditions of three microorganisms which could produce the EC precursor of hexanoic acid (HX) were optimized by uniform design and multi-factor interaction regression methodology in DPS data processing system. The results demonstrated that the trial data from this test fit the optimization model significantly,and the best optimized formula and temperature for the high-HX esterifying liquor were as follows: glucose 120 (g/L),yeast extracts 19 (g/L),MgCl2 1 (g/L),sodium acetate  27.5 (g/L),K2HPO4 1 (g/L) and 28 ℃. The optimized fermentation conditions were utilized to increase the production of HX esterifying liquid and the results of filling pit experiments by the esterifying liquid showed that the contents of EC and HX in distilled liquor in experimental group reached 622.37±35.74 and 416.26±18.91(mg/100mL) which represented 308.6%  and 197.24%  higher than that of the control group.

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Huang,Y.M.,Jian-Chun,L.U.,An-Jun,L.I.Analysis of flavoring substances in gujing gongjiu liquor by gc/ms. Liquor-Making Sci Technol,2006,7(145): 91-94.

Tang, L.Y., Guo-Hong, L. I., Wang, B. L., Wang, L.L., Wang, X. C. Improve the quality of luzhou-flavor liquor by useing yellow water esterified liquid. Food Ferm Technol, 2013,2013(3):50-51.

Chen, S., Liu, K. Y., Zheng, J., Jin, Y., Huang, J., Zhou, R. Q. Study on enzymatic esterification conditions of yellow water optimized by response surface methodology.Sci Technol Food Ind,2012,30(5), 400–405.

Liu Yigang, Zhou Zhiquan, Zhou Chao,et al.The application of whole-cell esterifying enzyme in bio-esterifying liquid [J]. Liquor-Making Sci Technol, 2016,No.2 (Tol.260):72-76.

Lan,Y.U. Improvement of the yield of quality kouzijiao liquor using esterified liquid produced by caproic acid bacteria. Liquor Mak, 2010,37(5):55~56.

Liu, B., Chen Yl., Yu Zl. Research on the preparation technology of esterifying liquid from yellow water. J Chin Inst Food Sc Technol,2009,9(2):116-121

Zhen, D., Wan, Z., Wang, J. B., Chen, M. B.Effects of yellow water and its content on the esterification of esterase. Liquor-Making Sci Technol, 2010,129(9):28-29.

Zhang D, Chen Xl, FuYu, Hu Xq, Wang Tao, You L. Application of a compound biological agent in the esterification of yellow water of Nongxiang Baijiu. Liquor-Making Sci Technol, 2016,267(9):71-75

Fang, K.T. and Zhang J.T. Criteria for uniform design, Technical Report Math-002,1992,Hong Kong Baptist College.

Kong F, Yu S, Bi Y, Huang X, Huang M. Optimization of Process Parameters and Kinetic Model of Enzymatic Extraction of Polyphenols from Lonicerae Flos. PHarma Mag,2016,12(45):70-74.

Buenno,L.H.,Rocha,J.C.,Leme,J.,Caricati,C.P.,Tonso,A. Use of uniform designs in combination with neural networks for viral infection process development. BIOTECHNOL PROGR ,2015,31(2), 532–540.

Xiong Jj, Zhang Yq, Zhou Cn etal. Optimization of Ginger Supercritical Extract Microcapsules Preparation Process by Uniform Design.Modern Chinese Med 2015,17(4):395-398.

Wang, J. P., Chen, Y. Z., Wang, Y., Yuan, S. J., & Yu, H. Q. Optimization of the coagulation-flocculation process for pulp mill wastewater treatment using a combination of uniform design and response surface methodology. water Res, 2011,45(17), 5633-5640.


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