DCC-mediated Inhibition of Progression in Breast Cancer

Abstract

DCC (deleted colorectal carcinoma) has been implied in several studies and play a major role in tumorigenesis. The present study evaluated the expression level of DCC in both breast cancer tissues and cell lines and the clinical significance of DCC in predicting the prognosis of breast cancer. The expression level of DCC mRNA was lower in both breast cancers tissues and cells with potential to metastasize. The Boyden chamber assay demonstrated that migration and invasion were decreased in MDA-MB-231 cells after upregulated DCC expression. The expression level of migration- and invasion-related genes,
MMP-1 and MMP-9, decreased obviously, while that of MMP-2, MMP-3, and MMP-7 was not affected. The level of TIMP1 was upregulated, while that of TIMP2 was unaffected. The level of DCC expression was lowered in high metastasis potential breast cancers in both cell lines and pathological tissues. The upregulated expression of DCC inhibits the migration and invasion of breast cancer cells by modulating the expression of MMP-1, MMP-9, and TIMP1.