Preparation of Factor VIII Using an Ion Exchange Medium CaptoTM MMC
Abstract
This article reported a method to prepare high quality of factor VIII using CaptoTM MMC. Results indicated that the specific activity of the FVIII eluate was varying between 40 and 90 IU/mg of protein. This means that this procedure could provide at least 102-to 230-fold purer FVIII compared with the original cryoprecipitation. The recovery rate was approximately 63.5%. The procedure is easily applicable to large-scale production of FVIII with acceptable recovery.
Keywords
Haemophilia, Factor VIII activity, Cryoprecipitation.
DOI
10.12783/dtbh/icmsb2017/17952
10.12783/dtbh/icmsb2017/17952
Refbacks
- There are currently no refbacks.