Neogenin Inhibits Migration, Invasion and Metastasis in Breast Cancer

Abstract

Neogenin is a multifunctional transmembrane glycoprotein, which can modulate cell migration by binding to its ligands and deliver intracellular signals. This study evaluated whether Neogenin is involved in the migration, invasion and metastasis of breast cancer. In this study, the expression level of Neogenin in breast cancer cells and tissues with different metastasis potentials were compared by immunohistochemistry, real-time PCR and western blot. Migration and invasiveness of MDA-MB-231 cells were tested by scratch wound model
and Boyden chamber assay after up-regulation of Neogenin expression. The expression of Neogenin mRNA was lower in breast cancer tissues and cells with higher metastasis potential. Scratch wound model and Boyden chamber assay showed that migration and invasion of MDA-MB-231 cells decreased after up-regulation of Neogenin expression. In conclusion, Neogenin expression was lower in breast cancer cell lines and pathological tissues with higher metastasis potential. Up-regulation of Neogenin expression inhibited migration and invasion
of breast cancer cells.