Effect of Exogenous Expression of VEGF165b on the Invasiveness of Human Bladder Cancer T24 Cells

Abstract

Objective To observe the effects of exogenous expression of VEGF165b on the
viability, migration rate and invasion ability of human bladder cancer T24 cells.and to explore the influence of VEGF165b in the biological constructed. The T24 cells were divided into T24 cells group (control), pcDNA3.0 group (transfected by pcDNA3.0 plasmid) and pc DNA-VEGF165b group (transfected by pcDNA-VEGF165b plasmid). The viability of T24 cell was detected by MTT assay; Western blotting method was used to determine the expressions of VEGF165b, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) proteins. Transwell assay was used to detect the rate of cell
migration and invasive ability. Results Compared with T24 cells group, the expression levels of VEGF165b,MMP-2 and MMP-9 proetins in the T24 cell in pcDNA3.0 group had na significant difference (P>0.05). Compared with T24 cells group or pcDNA3.0 group, the expression level of VEGF165b protein in the T24 cells in pcDNA-VEGF165b group was significantly increased (P<0.05). The cell viabilities of T24 cells had no significant difference between three groups (P>0.05). Compared with T24 cells group (migration rate 100%), the
migration rate of the T24 cells in pcDNA3.0 group (97.2%±7.8%) had no significant difference (P>0.05), but the migration rate in pcDNA-VEGF165b group was decreased significantly (63.5%±10.4%)(P<0.05).Compared with T24 cells group (70.8±8.5), the number of transferred T24 cells in pcDNA3.0 GROUP (66.3±11.2) had no significant difference (P>0.05), but in pcDNA-VEGF165b grou (23.5±7.3) it was decreased significantly (P<0.05). Conclusion Exogenous expression of VEGF165b in the T24 cells has no significant effect on the proliferation of T24 cells, but it can reduce the migration and invasion abilities
significantly.