DEStech Transactions on Biology and Health
Study on the Mathematical Relationship between the Fibonacci Sequence and Luoshu
In researches of basic theory of TCM, the Luoshu has direct relationship with the Jiugong bafeng theory. In this paper based on analysis of mathematical relations among the Fibonacci sequence, the Fibonacci rectangle and golden rectangle, according to geometric properties of the golden rectangle as well as numeric distribution rules implied in Luoshu, relationship between the Fibonacci sequence including its modular series by 10 and Luoshu is studied, and inherent and consistent mathematical relationship between them is found in spatial orientation, digital running sequence, numeric grouping and generating rules, etc. The study results of this paper can help to build up close mathematical association between Luoshu and the Fibonacci sequence, which can lay key theoretical foundation for the ultimate breakthrough for scientific mysteries containing in Luoshu and the Jiugong bafeng theory in TCM.
Efficacy of Swallowing Training Combined with Nutritional Intervention on Quality of Life in Laryngeal Cancer Patients with Dysphagia
Objective: To investigate the efficacy of swallowing training combined with nutritional intervention on quality of life in laryngeal cancer patients with dysphagia. Patients and Methods: A total of 66 patients who suffered from laryngeal cancer with dysphagia were selected and divided into control group (CG) and intervention group (IG), each of 33, according to random number tables. Both groups received total laryngectomy and prophylactic radiotherapy and were given routine health counseling and swallowing training, while intervention group was additionally provided with nutritional intervention. To evaluate all patients with Video Fluoroscopic Swallowing Examination (VFSE), Scored Patient-Generated Subjective Global Assessment on Nutritional Status (PG-SGA), and Quality of Life Questionnaire-core 30 (QLQ-c30) after radiotherapy and three months after. Results: VFSE, PG-SGA, QLQ-c 30 scores after swallowing training in IG were significantly greater than data collected instantly before swallowing training in IG and those collected after swallowing training in CG (P<.05). VFSE score after swallowing training in CG was significantly greater than data collected instantly before swallowing training (P<.05). Difference between before and after swallowing training on VFSE, PG-SGA, and QLQ-c 30 scores in IG were statistically significant greater than those of CG (P<.05). Conclusion: Swallowing training combined with nutritional intervention can improve swallowing function, nutritional status and quality of life in laryngeal cancer patients with dysphagia.
Efficacy of Comprehensive Rehabilitation on Quality of Life in Elderly Head and Neck Cancer Patients after Radiotherapy
Objective: To evaluate the effects of comprehensive rehabilitation on quality of life in elderly head and neck cancer patients after radiotherapy. Methods: Sixty-four subjects were randomly assigned to the rehabilitation group (RG) and control group (CG). Both groups were given routine healthy counseling, but RG was additionally provided with comprehensive rehabilitation. The depressive status, nutritional status and quality of life were assessed at the end of and three months after RT. Results: After comprehensive rehabilitation for three months, the patients in RG exhibited lower GDS, MNA-SF and higher QLQ-c30 score compared with those before rehabilitation and those in CG (P<0.05). The score of MNA-SF in CG at three months after RT was significantly better than those at the end of RT (P<0.05). Conclusion: The comprehensive rehabilitation can improve the depressive status, nutritional status and quality of life in older patients with head and neck cancer after radiotherapy.
26 Cases of Intractable Membranous Nephropathy Glucocorticoid Combined Gilad Amine Clinical Curative Effect Observation
To study the clinical effect of glucocorticoid combined with thalidomide in the treatment of refractory nephrotic syndrome with membranous nephropathy. Methods: the pathological for membranous nephropathy of 26 patients with refractory nephrotic syndrome, glucocorticoid + gilad amine 50 mg 2 times/d, to turn the urine protein application again after 12 weeks after the reduction of 50 mg, l/d, maintain half year outage. The invalid was given a maximum of 24 weeks to stop the drug. The observation time was 24 weeks. Results: 24h urine protein decreased from 9.15 ±3.23 to 1.43±1.91 (P<0.01).Plasma albumin rose from 26.02 ± 6.75 to 35.57 ±4.02 (P<0.01). In most cases, the onset time was between 8 and 16 weeks, with a total relief of 30.77% in 24 weeks and 50.00% in partial relief, with a total effective rate of 80.77%.The main side effects are: rash, lethargy, fatigue, dizziness. No liver and kidney damage, bone marrow suppression. Conclusion: thalidomide combined with glucocorticoid is effective in the treatment of membranous nephropathy, with mild side effects, which provides a new way for the treatment of refractory membranous nephropathy.
Clinical Research about Patients Who Use Lobaplatin During Breast Conserving Surgery
Objective to study the clinical effect of using Lobaplatin in breast conserving surgery. Methods: 40 patients who were eligible for breast conserving surgery were selected, and each group was divided into control group and experimental group. In the experimental group, the application of direct spraying and washing compatibility was applied to Lobaplatin, and the control group was washed with the same amount of physiological saline. After surgery, the blood image, liver and kidney function, digestive tract stimulation and wound healing were compared in the two groups, and the long-term metastasis and the recurrence rate were reviewed. Results No statistically significant difference (P> 0.05) in white blood cells and platelet count, serum creatinine and AST, ALT. The wound healing time was similar in the two groups, and there was no significant difference in nausea, vomiting and diarrhea. The recurrence rate in the experimental group was lower than that in the control group (P<0.05). There was no significant difference in distant metastasis. Conclusion: when using Lobaplatin in breast conserving surgery, there is no obvious side effect, obviously reducing the recurrence rate, it is worth popularizing widely.
Risk Factors and Intervention Measures of Occupational Exposure of Medical Staffs
Objective: To explore the risk factors and intervention measures of occupational exposure of medical staffs. Methods: The risk factors of occupational exposure were analyzed and targeted intervention measures were made according to professional characteristics of clinical work. Results: The protection consciousness and protection knowledge of medical and nursing staffs were improved. Conclusion: It is required to strengthen the training of occupational exposure knowledge for medical staffs and improve their understanding of occupational exposure. Based on the specific reasons for the existence of the problem, appropriate measures should be taken to improve the compliance of medical staffs, minimize the occupational exposure of health care workers and protect occupational safety of medical staffs.
Study on the Application of Undecylenamide Propyl Betaine and Polyhexamidine in the Incision of Breast Abscess
Objective: To evaluate the curative effect of incision and drainage of breast abscess and postoperative application of undecylen amidopropyl betaine and polyhexamidine (Prandtl liquid dressing and wound gel). Methods: 100 cases of breast abscess, 50 cases of observation group and control group were randomly selected. The observation group was treated with undecyl amido propyl betaine and polyhexamethylene biguanide (Prandtl liquid dressing and wound gel) Drainage patients were dressing change, the control group with saline for dressing, t test was used to compare the incision healing time, the number of dressing, using the χ2 test to compare the recurrence rate. Results: The incision healed completely in both groups. Compared with the control group, the wound healing time in the observation group was shorter, the dressing frequency was lower and the recurrence rate was lower (all P <0.05). Conclusion: After incision and drainage of patients with breast abscess, dressing with undecylenamide propyl betaine and polyhexamidine (prontosan wound irrigation solution and wound gel) can shorten the wound size after incision and drainage of breast abscess Healing time, reduce the frequency of dressing, while reducing the recurrence rate.
The Influence of Empathy Nursing on the Improvement of the Negative Emotions of Young Breast Cancer Patients
Objective: Discuss the influence of empathy nursing on the negative emotions of young breast cancer patients. Method: 132 young breast cancer patients admitted from February 2016 to February 2017 were selected and divided into observation group (66 patients) and control group (66 patients). The control group was given routine nursing, while the observation group was given empathy nursing based on routine care. SAS (Self-Rating Anxiety Scale) and SDS (Self-Rating Depression Scale) were adopted to analyze the influence of the two nursing methods on the negative emotions of the two groups. Results: Before nursing, by comparing the scores of SDS and SAS of the two groups, P>0.05, which means there is no statistically significant difference; after the nursing, the scores of SAS and SDS of the two groups decreased, and the degree of decline in the control group was less than the observation group, the difference is statistically significant. Conclusion: Empathy nursing can effectively improve the negative emotions of young breast cancer patients and is beneficial to the recovery.
The Effect of Arita in Postoperative Drainage of Patients with Breast Cancer Modified Radical Surgery
Purpose: To study the application of Arista in breast cancer modified radical surgery, whether there is any effect on postoperative drainage. Methods: To collect the data of patients who have undergone breast cancer modified radical surgery in our hospital within two years; It was divided into control group, single dose group, double dose group and multi-dose group, and compared with those in the four groups. Results the flow rate of the single dose group was faster than that of the control group, and the double-dose group was better than that of the single dose group, and there was no significant difference between the two groups. Conclusion in the treatment of breast cancer modified radical operation, it can significantly reduce the tissue exudation and reduce the induced flow, and the overuse does not have a better effect.
Use of Undecylenamide Propyl Betaine and Polyhexamidine in the Treatment of Mammary Abscess with Mammotome System
Objective: To investigate the application value of elemene amidopropyl betaine and polyhexamidine (Prontosan wound irrigation solution) in the treatment of breast abscess with Mammotome system and abscess incision and drainage. Methods: Totally 80 breast abscess patients treated in our hospital were randomly divided into 2 groups, which were divided into two groups: the control group and the control group. The two groups were treated with undecylenamide propyl betaine Polygonum biguanide (Prontosan wound irrigation solution) repeated washing, and give the conventional dressing, t test was used to compare the two groups of wound healing time, dressing change, dressing pain situation, the use of 2 test to compare the two groups of patients relapse rate . Results: Compared with incision and drainage group, the wound healing time was shorter, the number of dressings was less, the degree of dressing change was lighter, the relapse rate between two groups was higher than that of the control group low.
Efficacy of Aidi Injection Combined with Chemotherapy in the Treatment of Advanced Epithelial Ovarian Cancer
Objective: To study the efficacy, quality of life and side effects of Aidi injection combined with paclitaxel and cisplatin in the treatment of advanced epithelial ovarian cancer. Methods: 68 cases of advanced epithelial ovarian cancer were randomly divided into 2 groups, observation group and the control group, each group contains 34 cases. The patients in the control group were treated with chemotherapy, and the patients in the observation group were treated with Aidi injection combined with chemotherapy. We evaluated the efficacy and toxic and side effects in every 2 treatment cycles. Result: The effective rate of the patients in the observation group had no different with that of the control group (64.7% to67.6%)(P>0.05),the improvement of quality of life in the observation group was better than that of the control group, and the difference was statistically significant (P<0.05),the incidence of leukocyte decline, abdominal pain and nausea in the observation group were significantly lower than those in the control group (P<0.05), and the difference was statistically significant. Conclusion: Aidi injection combined with paclitaxel and cisplatin is safe and effective in the treatment of advanced epithelial ovarian cancer. Compared with chemotherapy alone, it improves the life and does not increase toxicity.
A Comparison of the Effect of Caebetocin and Ergonovine Combination with Oxytocin in Preventing Postpartum Hemorrhage
Objective To comparison of the effect of caebetocin and ergonovine combination with oxytocin in Preventing Postpartum Hemorrhage. Methods:120 full term pregnant women in the China-Japan Union Hospital of Jilin University were selected as the subjects of this study from June 2015 to December 2017.It was divided into the observation group (Group A) and the control group (Group B)according to the random digital table method, with 60 pregnant women in each group. Record the general information of pregnant women, the amount of bleeding of parturients, 2 hours and 12 hours after delivery and the change of hemoglobin, the times of contraction, the systolic pressure, and the diastolic pressure. Result There was no statistically significant difference in the general data (age, gestational week, birth weight) between the two groups of parturients. The amount of bleeding, the change of hemoglobin and the number of uterine contraction in the observation group were significantly better than those in the control group, P<0.05. . There was no significant difference in systolic and diastolic pressure between the two groups, P>0.05. Conclusions: The effect of using carbetocin to prevent postpartum hemorrhage is more significant than ergonovine combination with oxytocin, and can guarantee the safety of mothers, improve maternal prognosis, it is worthy of clinical application.
Significance and Changes of AGR2 Level in Tissue and Serum of Patients with Cervical Cancer Before and After Operation
Objective To detect the changes of anterior gradient-2 (AGR2) level before and after operation in tissue and serum of patients with cervical intraepithelial neoplasia (CIN) and cervical cancer, and to explore its clinical significance. Methods the serum and tissue of patients with cervical cancer (n=30) and patients with CIN (n=20) before and after operation were collected. Serum sample from 20 health women and cervix tissue samples of 20 patients undertaken total hysterectomy due to myoma were collected for the control group. AGR2 levels were detected by enzyme-linked immunosorbent assay (ELISA). Results the levels of AGR2 in preoperative serum and tissues of cervical cancer were significantly higher than that of CIN and normal control group (all P<0.01); the levels of serum AGR2 after operation in the cervical cancer and CIN patients were significantly lower than that of preoperative (all P<0.01); the AGR2 levels of serum and tissue of cervical cancer patients were significantly different for the tumor size, FIGO stages and lymph node involvement (P<0.01 or 0.05); Pearson analysis showed that serum AGR2 levels before operation positively correlated to that after operation in the patients with CIN and cervical cancer (r=0.747, P=0.000); the levels of serum AGR2 before and after operation positively correlated to that in cervical tissue (r=0.789, P=0.000; r=0.700, P=0.000). Conclusion the levels of AGR2 in serum and tissue of cervical cancer increased obviously, which closely related to tumor size, FIGO stages and lymph node involvement. It is valuable to detect the level of AGR2 for judging the surgical curative effect of patients with cervical cancer.
Intraoperative Nursing Cooperation for Electromagnetic Image-Guided Endoscopic Sinus Surgery
Purpose: Endoscopic sinus surgery is nowadays routinely used in chronic rhinosinusitis, the technique now has expanded and used to treat orbital and skull base diseases. However, the complications including severe bleeding, blindness and injure to the central nervous system have been reported once in a while. To address these concerns, electromagnetic navigation system has been used in endoscopic sinus surgery at home and abroad. The navigation system is a computer aided technique that helps identify important anatomical structures by using three-dimensional image information. Surgeons could get more intuitive and more detailed imaging information in the operative field (anatomical structures “mapâ€), identify the relationships between structures and surgical instruments conveniently, thus preventing and reducing endoscopy-related issue. Navigation system through an endoscopic sinus surgery was to be a significant part of nasal basic research and clinical practice; although there’re limitations, such as cannot provide intraoperative changes on real time and the heavier economic burden. Intraoperative nursing cooperation for electromagnetic image-guided endoscopic sinus surgery will become increasingly important.
Observation on the Effect of Uterine Artery Embolization Combined with Hysteroscopy in the Treatment of Cesarean Scar Pregnancy
Objective To study the effect of uterine artery embolization (UAE) and methotrexate (MTX) combined with hysteroscopy in the treatment of cesarean scar pregnancy(CSP). Methods:82 cases of cesarean scar pregnancy in the China-Japan Union Hospital of Jilin University were retrospectively studied between January, 2010 and January, 2016.They were divided into the two groups on the treatment method. The group A was offered prophylactic uterine artery embolization (UAE) and methotrexate (MTX) prior to uterine curettage with hysteroscopy method. The group B was treated with mifepristone prior to uterine curettage with hysteroscopy as routine therapy. The effect of the two groups after treatment was analyzed and compared. Results: The blood loss of the group A (27.42 ± 15.31)mL was significantly lower than that of the group B, P<0.05.The total effective rate of the group A was 97.24%,which was significantly higher than that of the group B of 69.86%, P <0.05. The bleeding days in the group A were significantly shorter than those in the group B, P <0.05. The surgical time of group A was significantly shorter than the group B. Conclusion: Uterine artery embolization combined with hysteroscopy in the treatment of cesarean scar pregnancy can significantly improve the bleeding situation and prognosis.
A Comparative Study of Lobaplatin and Cisplatin in the Treatment of Advanced Ovarian Cancer Undergoing Hypertherimic Intraperitoneal Chemotherapy Combined with Intravenous Chemotherapy
Purpose: To explore and compare the curative effect and side effects of the patients with epithelial cell ovarian carcinoma after surgery administered by hyperthermic intraperitoneal chemotherapy (HIPEC) with lobaplatin combined with intravenous chemotherapy. Method: Forty patients with epithelial cell ovarian carcinoma treated by HIPEC and systemic chemotherapy after CRS were selected from January 2012 to September2014, and were divided into two group, Group Lobaplatin and Group Cisplatin, according to the kind of platinum used in HIPEC. The general information, 1,3-year survival rate, serum CA125 level and adverse events were recorded. Result: As for the 1,3-year survival rate, serum CA125 level, there is no statistical difference comparing data of two groups. As for adverse events between two groups, the occurring of nausea, vomiting and abdominal pain of the lobaplatin group is significantly lower than that of the cisplatin group. Conclusion: Hyperthermic intraperitoneal chemotherapy with Lobaplatin in combination with intravenous chemotherapy is effective and feasible treatment for patients with advanced ovarian carcinoma. Lobaplatin and cisplatin both have acceptable complication rate and survival rate. According to our study, patients in lobaplatin group have better compliance than cisplatin.
A Retrospective Analysis of the Risks of Intraductal Lesions in Patients without Pathological Nipple Discharge (PND)
In order to explore the risk factors of intraductal lesions in patients without pathological nipple discharge. Retrospective analysis of the clinical and pathological data of the 350 Intraductal lesion cases that had underwent breast surgery in the China-Japan Union Hospital of Jilin University from April 2015 to November 2018. There were 216 patients with unilateral lesions and 67 patients with bilateral lesions. This study included133 cases with pathological nipple discharge (PND) and 217 cases without nipple discharge (non-PND). The composition ratio of different pathological types was analyzed between the group with PND and the group without PND. 217 non-PND intraductal lesion cases with full clinicopathological information were chosen for further analysis. In order to analyze the relative risk factors of intraductal lesion in patients without PND, clinical parameters were evaluated by Chi-Square test and multivariate Logistic regression analysis. Ageâ‰¤50 years old (OR, 0.218; 95% CI, -3.038- -0.011) and BIRADS gradeâ‰¤3 (OR, 0.021; 95% CI, -5.192- -2.527) are the protective factor of intraductal lesions. Single nodule (OR, 16.461; 95% CI, 1.948-3.655) is the relative risk factor of intraductal lesions.
Thermal Sensory Analysis of Cervical and Thoracic Temperature Sensibility in Female with Breast Pain
In order to distinguish the grades of breast pain with a quantitative index, the detection and pain thresholds were measured with the technique of TSA (Thermal Sensory Analysis) on patients with breast pain. We aimed to explore whether TSA could be used to predicate the degree of breast pain as an objective indicator or not. Thirtytwo female outpatients with breast pain were included in the study by inclusion criteria. A total of sixtyfour breasts were measured. The cold pain threshold (CPT), cold detection threshold (CDT), warm detection threshold (WDT) and heat pain threshold (HPT) were measured at the following five points of both sides: neck, shoulder, axilla, back and super ior lateral quadrant of breast. The sixtyfour breasts were devided into three groups: Group A consisted of 29 breasts that have breast pain grade from 0 to 1 (No pain at that time); Group B consisted of 20 breasts that have breast pain grade from 2 to 4 (Pain exist, but participant can’t give it an explicit score); Group C consisted of 15 breasts that have breast pain grade from 5 to 7. Statistic analysis was performed by SPSS 20.0 to compare the means and SD. Variance for each threshold at each site was expressed as Coefficient of Variation percentage (CV%), using the standard deviation (SD) divided by the meanx100. On every sites, group B has the highest value of WDT and HPT. And expect the site of axilla, group C has higher values than group A of WDT and HPT. On the site of breast, the values of CPT and CDT have the trend to increase from group A to group C. But the differences in groups were without statistically significant differences (p>0.2). The CV% of HPT and WDT are lowest and near to each other, and CPT has the highest value of CV%. Breast pain can not only reduce the sensibility of warm detection and heat pain of breast, but also the sites close to breast. Oppositely, breast pain can enhance the sensibility of cold detection and cold pain of breast. But C PT has the highest value of CV%, this may means CPT has poor reliability due to wide variance.
Efficacy of Fractional Carbon Dioxide Laser Followed by Ultrasound Introduction of Diprospan on Vitiligo
The aim of this study is to evaluate the efficacy of the fractional CO2 laser in combination with ultrasound introduction of diprospan on the treatment of vitiligo. Effective rate and repigmentation time were significantly better in combining treatment group than fractional laser group and tacrolimus group (P<0.05), suggesting that fractional CO2 laser combined with ultrasound introduction of diprospan has more significant therapeutic effect over fractional CO2 laser or tacrolimus only. In addition, curative effect of faciocervical lesion was much better than of truncal lesion in all three groups after treatment. Noticeable adverse events were not found in any patient in this study. The results demonstrate that fractional CO2 laser therapy followed by ultrasound introduction of diprospan could be used effectively and safely as an alternative modality for the treatment of vitiligo.
Neogenin Inhibits Migration, Invasion and Metastasis in Breast Cancer
Neogenin is a multifunctional transmembrane glycoprotein, which can modulate cell migration by binding to its ligands and deliver intracellular signals. This study evaluated whether Neogenin is involved in the migration, invasion and metastasis of breast cancer. In this study, the expression level of Neogenin in breast cancer cells and tissues with different metastasis potentials were compared by immunohistochemistry, real-time PCR and western blot. Migration and invasiveness of MDA-MB-231 cells were tested by scratch wound model and Boyden chamber assay after up-regulation of Neogenin expression. The expression of Neogenin mRNA was lower in breast cancer tissues and cells with higher metastasis potential. Scratch wound model and Boyden chamber assay showed that migration and invasion of MDA-MB-231 cells decreased after up-regulation of Neogenin expression. In conclusion, Neogenin expression was lower in breast cancer cell lines and pathological tissues with higher metastasis potential. Up-regulation of Neogenin expression inhibited migration and invasion of breast cancer cells.
Role of Neogenin in Regulating Proliferation and Apoptosis in Breast Cancer
In our former study we have published that the expression of Neogenin wasreduced in both breast cancer cells and breast cancer tissues. The aim of this study was toexplore the role of Neogenin in regulating proliferation and apoptosis in breast cancer. Firstly,Neogenin expression in MDA-MB-231 cells was up-regulated by usingpcDNA3.1-eGFP-Neogenin plasmids. The results of transfection were detected by fluorescentmicroscope observation, real-time PCR test and western blot. Then the proliferation andapoptosis of MDA-MB-231 cells after Neogenin being up-regulated were tested by usingCCK8 assay, flow cytometry assay and TUNEL staining. CCK8 results showed thatMDA-MB-231 cells proliferated more quickly after transfected bypcDNA3.1-eGFP-Neogenin while compared with pcDNA3.1-eGFP and MDA-MB-231groups (P<0.01). Flow cytometry assay showed that there were fewer cells in S phase of cellcycle in pcDNA3.1-eGFP-Neogenin group and more cells in G0/G1 phase while comparedwith other two groups(P<0.05).TUNEL test showed that the apoptosis rate inpcDNA3.1-eGFP-Neogenin group was significantly higher than other two groups(P<0.05).We found that up-regulating Neogenin expression would inhibit proliferation and promoteapoptosis in MDA-MB-231 cells. However, further studies are still needed to explore themechanism of these variations in proliferation and apoptosis.
Pathological Analysis of 2865 Cases of Intraductal Papillary Lesions by Ductoscopy
The aim of this study is to explore the pathological analysis of 2865 cases of intraductal papillary lesions by ductoscopy and to discuss the features of ductoscopicappearance of malignant cases. We reviewed 2865 papillary lesions diagnosed using ductoscopy from April 23, 2013 to October 31st, 2017 who were evaluated by using surgical excision biopsy and pathology. Data concerning patient age, discharge and the details of endoscopic appearance were analyzed according to the pathological results. A total of 2715 patients complained of pathological nipple discharge (PND) were collected. The postoperative diagnosis in 2290 patients (79.9%) was benign and 260 patients (9.08%) was ADH. A total of 315 (11.0%) cases were found to be malignant. In our study, on univariate analysis, patients of with spontaneous and bloody discharge were more likely to suffer from malignant lesions. On logistic regression analysis, bloody nipple discharge, morphology, and a broad lesion base revealed by ductoscopy showed a statistically significant correlation with malignancy (p<0.05,respectively). The endoscopic features of bloody discharge, morphology, and a broad lesion base are independent risk factors for malignancy and represent new criteria for the diagnosis of patients with PND.
Progress in CD80 and Glomerular Micropathy
Small lesion is a common pathological type nephrotic syndrome, especially in high-risk children; common complications include infection, thrombosis, and acute renal failure and protein and lipid metabolism disorders. The pathogenesis of this disease is still not clear, and the current research focus has been turned to the study of foot cells by the study of T cell immune disorders. However, CD80 is expressed in the foot cells in small glomerular lesions, and is related to the formation of egg white urine. This article reviewsthe relationship between CD80 and glomerular micropathy.
Progress in the Mechanism of IgA Nephropathy Renal Injury
IgA nephropathy is the most common primary glomerular disease in the world, accounting for about 25% ~50% of primary glomerular diseases [1]. Clinical IgA nephropathy occurs in young and middle-aged adults, and about 30-40% of patients enter the end-stage renal failure (ESRD) in 10-20 years[2], which brings a heavy burden to the country and society. IgA nephropathy pathogenesis is not clear at present, has long been a mesangial cells is considered to be major cell types of the disease, IgA nephropathy and recent study found that IgA nephropathy mesangial cells and sertoli cell and the interaction between the tubular epithelial cells, the common cause of kidney damage of IgA nephropathy [3-5]. 2009 international IgA nephropathy group proposed IgA nephropathy Oxford disease classification, it included four indicators of clinical outcomes with independent predictive value, namely, mesangial matrix (M), segmental sclerosis (S), endothelial cell hyperplasia (E) and tubular atrophy and interstitial fibrosis (T) [6]. Has rainbow multicenter IgA nephropathy as verification of Oxford's pathological classification in China, M, S, T three pathological indexes closely related to long-term renal outcomes for patients with IgAN, but E no obvious correlation with long-term prognosis. Therefore, mesangial cells, foot cells and tubule epithelial cells play an important role in the development of IgA nephropathy. In this paper, combined with domestic and foreign literature on mesangial cells, sertoli cell and tubular epithelial cells of IgA nephropathy renal injury mechanism, to clarify to the pathogenesis of IgA nephropathy[7], and clinical diagnosis and treatment to provide new inspiration point and basis.
Research Progress on the Correlation Between SLE and Malignant Tumor
Systemic lupus erythematosus (SLE) is a prototypical systemic autoimmune disease, characterized by a wide array of symptoms and organ involvements, leading to varying disease courses and outcome, and ranging from mild to severe types. In patients with SLE, the incidence and risk of malignancy development is increased, and non-Hodgkin's lymphoma (NHL), cervical cancer, as well as bronchial carcinomas occur, are common malignancy types. Common genetic predisposition, chronic antigen stimulus, disproportional immune responses, as well as the chronic administration of immunosuppressive medications can contribute to the development of malignancies in SLE patients. In this review we present some research areas such as the molecular pathology, epidemiological and clinical of the malignancies in SLE patients population.
Intravenous Thrombolytic Therapy for Deep Venous Thrombosis of the Lower Extremities
Objective to investigate the efficacy and clinical application of catheter thrombolysis in the treatment of acute deep venous thrombosis of the lower extremities. Patients with acute lower limb DVT 2016 November 2013 year in November 50 cases as experimental group, June 2007 to 2010 December were acute lower extremity DVT patients 69 cases as control group. All patients were diagnosed by venography of lower extremity or Doppler by intravenous ultrasound, and symptoms such as pain or tenderness in the groin area, pain of the limb and so on. Results the clinical curative effect of 12 groups of test group 50 cases, 1 cases were lost, 13 cases were cured, 34 cases markedly effective in 2 cases, the total efficiency of 98% (49 /50); the control group of 69 cases, 11 cases were cured, 26 cases markedly effective, 5 cases effective, 27 cases ineffective, the total effective rate was 60.9% (42 /69).
Study on the Correlation of Diabetic Lower Extremity Artery Disease and Prognosis
Studies have shown that diabetic feet occur in 15%~25% diabetics, and about half of them will have wound infection. There are many factors that lead to poor prognosis of diabetic foot, such as peripheral vascular disease, area and depth of foot ulcer, peripheral neuropathy and infection. Therefore, the pathogenesis of diabetic foot is complex, and many factors are one of the main causes leading to amputation. At the same time, diabetic foot ulcers can significantly increase the health risk of diabetic patients; increase the incidence of cardiovascular accidents and all-cause mortality. Therefore, the early diagnosis and treatment of diabetic foot is an important means to improve the quality of life and the survival time of diabetic patients. Although the treatment of diabetic foot has achieved remarkable results, because of its complex mechanism, the existing treatment methods are still difficult to meet the clinical needs, and new therapies need to be further explored. In our hospital from April 2014 to August 2016 a total of 67 cases of diabetic patients as the research object, which will be 36 cases of diabetic foot were classified as group DF, 31 cases of non-diabetic foot patients were classified as non DF group, compare two groups of patients with lower limb artery disease, 1 years of follow-up will be based on whether the amputation patients divided into amputation group and non-amputation group, and the general situation and related factors of two groups of patients were statistically analyzed.
Effect of Exogenous Expression of VEGF165b on the Invasiveness of Human Bladder Cancer T24 Cells
Objective To observe the effects of exogenous expression of VEGF165b on the viability, migration rate and invasion ability of human bladder cancer T24 cells.and to explore the influence of VEGF165b in the biological constructed. The T24 cells were divided into T24 cells group (control), pcDNA3.0 group (transfected by pcDNA3.0 plasmid) and pc DNA-VEGF165b group (transfected by pcDNA-VEGF165b plasmid). The viability of T24 cell was detected by MTT assay; Western blotting method was used to determine the expressions of VEGF165b, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) proteins. Transwell assay was used to detect the rate of cell migration and invasive ability. Results Compared with T24 cells group, the expression levels of VEGF165b,MMP-2 and MMP-9 proetins in the T24 cell in pcDNA3.0 group had na significant difference (P>0.05). Compared with T24 cells group or pcDNA3.0 group, the expression level of VEGF165b protein in the T24 cells in pcDNA-VEGF165b group was significantly increased (P<0.05). The cell viabilities of T24 cells had no significant difference between three groups (P>0.05). Compared with T24 cells group (migration rate 100%), the migration rate of the T24 cells in pcDNA3.0 group (97.2%±7.8%) had no significant difference (P>0.05), but the migration rate in pcDNA-VEGF165b group was decreased significantly (63.5%±10.4%)(P<0.05).Compared with T24 cells group (70.8±8.5), the number of transferred T24 cells in pcDNA3.0 GROUP (66.3±11.2) had no significant difference (P>0.05), but in pcDNA-VEGF165b grou (23.5±7.3) it was decreased significantly (P<0.05). Conclusion Exogenous expression of VEGF165b in the T24 cells has no significant effect on the proliferation of T24 cells, but it can reduce the migration and invasion abilities significantly.
The Application of the Inferior Vena Cava Filter in the Prevention of Secondary Pulmonary Embolism of Deep Venous Thrombosis of the Lower Extremity
To investigate the effect and safety of inferior vena cava filter (VCF) implantation in the prevention of secondary pulmonary embolism (DVT) of lower extremity deep venous thrombosis (DVT). Methods: To observe 225 patients received VCF implantation combined with thrombolytic therapy, the clinical efficacy and complications of simple catheter directed thrombolysis and systemic thrombolysis in treatment of patients with DVT incidence, and monitoring of oxygen saturation (Sp O2) and arterial oxygen pressure (Pa, O2) were secondary pulmonary embolism (PE) and the occurrence of recurrent thrombosis after treatment. Results: the difference of peripheral circumference between healthy and disabled group was significantly reduced after combined treatment and catheter thrombolysis (P< 0.05). There were 5 cases of hematuria, 4 cases of hemoptysis and 2 cases of gastrointestinal bleeding in systemic thrombolysis group, and no serious complications occurred. Conclusion: VCF implantation can effectively improve the efficacy and safety of DVT, and it has important clinical value.
Clinical Study of Low Dose Urokinase Combined with Low Molecular Weight Heparin in the Treatment of Deep Venous Thrombosis of the Lower Extremities
To observe the curative effect of slow infusion of low-dose urokinase and low molecular weight heparin calcium in elderly patients with acute deep venous thrombosis. Methods: 45 patients with acute deep venous thrombosis in the lower extremities were selected and randomly divided into two groups. 20 cases of the control group using the traditional method of administration by the limbs of other body superficial vein infusion of small dose urokinase (400000uêžtimes-1,1timeêžd -1) thrombolysis and low molecular weight heparin (100uêžkg-1, Ih, 1timeêž12 h-1) on the first day of anticoagulation. Play with warfarin 2.5 mg - late 1 (adjusted according to INR dosage, INR 2~3) for 14 days; 25 patients in the experimental group improved drugs were given by the distal limb superficial vein slow pump into the small dose of urokinase (100000uêžtimes-1, 2timesêžd-1), low molecular weight heparin calcium (100 Uêžkg-1, Ih, 1timeêž12 h-1), the first day with warfarin 2.5 mg - late 1 (adjusted according to INR dosage, INR 2~3) for 14 days. Results: in the control group, 2 cases were cured, 7 cases were improved, 11 cases were invalid, 9 cases were total effective, 11 cases were cured in the experimental group, 9 cases were improved, 5 cases were invalid, 20 cases were total effective. The total effective rate of the two groups was statistically different (P = 0.03). Conclusion: the improved method of slow infusion of small dose of urokinase and low molecular heparin calcium through the distal superficial veins of the affected limb in elderly patients with deep venous thrombosis is superior to the traditional medication.
The Roles of Exosomes Related to Some Oral Diseases
Exosomes are nanometer-sized, membranous vesicles secreted by many cell types into surrounding extracellular space and body fluids. In recent years, studies have shown that exosomes play important role in many physiological and pathological processes such as immune regulation, cell differentiation, infection and cancer. As an efficient mediator of intercellular communication, exosomes have been demonstrated to widely exist in oral fluid and supernatants. Meanwhile, the important roles of exosomes in some oral diseases have also been revealed. This review aims to introducing characteristics of exosomes in oral cavity, summarizing their functions in different physiological and pathological conditions and provides the further insight into potential application of exosomes to disease diagnosis and treatment.
DCC-mediated Inhibition of Progression in Breast Cancer
DCC (deleted colorectal carcinoma) has been implied in several studies and play a major role in tumorigenesis. The present study evaluated the expression level of DCC in both breast cancer tissues and cell lines and the clinical significance of DCC in predicting the prognosis of breast cancer. The expression level of DCC mRNA was lower in both breast cancers tissues and cells with potential to metastasize. The Boyden chamber assay demonstrated that migration and invasion were decreased in MDA-MB-231 cells after upregulated DCC expression. The expression level of migration- and invasion-related genes, MMP-1 and MMP-9, decreased obviously, while that of MMP-2, MMP-3, and MMP-7 was not affected. The level of TIMP1 was upregulated, while that of TIMP2 was unaffected. The level of DCC expression was lowered in high metastasis potential breast cancers in both cell lines and pathological tissues. The upregulated expression of DCC inhibits the migration and invasion of breast cancer cells by modulating the expression of MMP-1, MMP-9, and TIMP1.
Clinical Application Research on the Use of Prontosan Wound Irrigation Solution in Minimally Invasive Treatment of Breast Abscess Patients
Study on the effect of Prontosan wound irrigation solution in patients undergoing breast abscess minimally invasive surgery. 62 cases of breast abscess patients undergoing Minimally Invasive Surgery were randomly divided into treatment group (33 cases) and control group (29 cases). The treatment group received Prontosan wound irrigation solution flushing and the control group received physiological saline flushing. The wound healing time, postoperative drainage, postoperative hospital stay and times of dressing change were collected for statistical analysis. There were significant differences in the wound healing time, postoperative hospital stay and times of dressing change between treatment group and control group (P<0.05). There was no significant difference in postoperative drainage between study group control group (P>0.05). Prontosan wound irrigation solution is effective in promoting wound healing, shortening postoperative hospital stay and reducing the times of dressing change. There were no side effects observed in the process of using Prontosan wound irrigation solution. It is worth in clinical application.
NMR-based Metabonomic Studies on Intervention Effects of Zuojin Pill on Stomach Heat Syndrome in Rats
Zuojin Pill (ZJP), composed of Rhizoma Coptidis and Fructus Euodiae, is commonly used for the treatment of stomach heat syndrome (SH) in traditional Chinese medicine, but the underlying molecular mechanisms remain unclear. The present study was performed to investigate the protective effect and the potential mechanisms of ZJP at metabolic level in a rat model of SH. Proton nuclear magnetic resonance (1H NMR) based metabonomic approach was developed to profile SH-related metabolic perturbations in rat urine and feces. Distinct clustering and a clear separation of the model group from control and ZJP groups was observed. Furthermore, seven and five potential biomarkers associated with SH in rat urine and feces, respectively, which were mainly involved in energy metabolism and gut microbiota metabolism, were identified. ZJP could reverse the pathological process of SH through regulating the perturbed metabolic pathways. These findings offered new insights into in-depth understanding of the pathogenesis of SH and laid scientific foundation for the traditional use of ZJP in treating SH-related gastrointestinal diseases.
Safety Problems and Intervention Measures of Medication in Chinese Elderly
As a special group in China, the elderly accounted for relatively large. The problem of rational drug use has become a focus in the society. The elderly are in the aging state in physiological, psychological and other aspects. As the prevalence increases, drug abuse increases and the consequent adverse reactions are endangering the health of the elderly. This paper analyzed the safety problems of medication in the elderly and explores safe, effective and individualized interventions, so as to provide a reference for clinical decision making.
The Effects of Memory Rehabilitation in Patients with Vascular Dementia
This study is mainly to use memory nursing rehabilitation training method to intervene vascular dementia patients, observe the changes of memory function of patients, and find evidence of evidence-based medicine for the effectiveness of memory training rehabilitation nursing intervention vascular dementia. The patients were divided into the experimental group and the control group based on the selection of the patient and the families, 20 cases in each group. The control group was given routine treatment, the experimental group also performed rehabilitation treatment for the patient’s memory except routine treatment, including association method, recitation method, decompose - association method, reminder method, memory technique and other memory training. The specific operation methods include visual memory, map work, and block arrangement of colored blocks, memory treatment and so on. The training once per day, 60 minutes every time for a month, two groups of patients use hasegawa dementia scale (HDS), wechsler memory scale (WMS) for quality evaluation, and use Paired t test compare the memory differences between the two groups. The average score of HDS scale between experimental and control group were 26.18±3.92 and 18.16±3.11 after the intervention, respectively. The average score of WMS scale between two groups were 84.16±5.15 and 62.13±4.12 after the intervention, respectively. The memory rehabilitation training could help improve the memory of vascular dementia.
Research Progress of Care Bundles in Nursing Practice
Evidence-based nursing has been introduced into China for more than ten years, and many researches have been conducted on evidence-based nursing in recent years. Based on the background of this clinical research, care bundles were put forward in 2001, and then there was an endless stream of research on cluster nursing. The purpose of this study is to expound the clinical practice of care bundles application at home and abroad, and to understand the general principles and characteristics of cluster nursing, so as to provide reference for future research.
Research Progress of Aerobic Endurance Training on Cardiac Rehabilitation in Patients with Chronic Heart FailureResearch Progress of Aerobic Endurance Training on Cardiac Rehabilitation in Patients with Chronic Heart Failure
Heart failure is due to any abnormal cardiac structure or function of the ventricular filling and (or) ejection ability damage caused by a group of clinical syndromes, the main symptoms were dyspnea, fatigue and fluid retention, chronic heart failure (CHF) is the final destination of most cardiovascular disease, also is the main cause of death[1].In 2016, the Chinese cardiovascular disease report mentioned 4.5 million patients with heart failure in China, and the death rate of 5.4% in 30 days [2].Patients with CHF had poor prognosis, high mortality and poor quality of life due to their long duration, multiple complications and poor compliance with medication. The prescription of cardiac rehabilitation exercise is important for the improvement and prognosis of CHF symptoms. Cardiac rehabilitation includes aerobic endurance training, flexibility training, resistance training, and aerobic endurance training as the basis for a heart rehabilitation program. This paper will summarize the clinical evidence of aerobic endurance training to improve the prognosis of CHF patients, and aim to help the development of aerobic endurance training in China.
The Effects of Rehabilitation of Life in Patients with Care Bundle for Stroke Swallowing Dysfunction
The propose of this study was to explore the effect of care bundle on patients with dysphagia. This study adopt randomized controlled trial, patients were divided into two groups, the control group was given routine treatment, the experimental group was implemented care bundle measures on the basis of routine rehabilitation nursing. The daily training for 1 month, two groups of patients were evaluated by the correlation scale before and after nursing. The average score of Barthel scale between experimental and control group were 85.4±4.1 and 71.3±3.0 after the intervention, respectively. The swallowing dysfunction of experimental group after intervention were better than the control group. The average score of the quality of life scale between experimental and control group were 92.4±5.9 and 81.3±6.5 after the intervention, respectively. The Care bundle is of great clinical significance in improving the rehabilitation effect of patients with dysphagia.
Application of Rehabilitation Nursing in Patients with Vascular Dementia
This study mainly discusses the application of rehabilitation nursing in patients with vascular dementia, and to find evidence of the efficacy of rehabilitation nursing intervention in the treatment of vascular dementia. Sixty patients with vascular dementia were randomly divided into observation group and control group. Patients in the control group were given routine care. Observation group of patients on the basis of conventional rehabilitation nursing, psychological nursing, intelligent training, functional rehabilitation training), 4 weeks by CDR, WMS, ADL, FAQ, on two groups of patients on the improvement of their cognitive ability and the ability to care for daily life. The results show that rehabilitation nursing for patients with vascular dementia can effectively improve patients’ cognitive ability and daily self-care ability. The nursing model is worthy to be widely promoted in clinical practice to help more patients benefit.
Catalyzed Synthesis and Cytoprotective Activity of 5-(furan-2-yl)-3-Phenyl-Isoxazole
In this work, 5-(furan-2-yl)-3-phenyl-isoxazole was synthesized by catalyzed by CaO as solid basic catalyst. CaO showed great catalytic activity to the condensation reaction between hydroxylamine and chalcone, giving 78.5% yield of target product at ratio of hydroxylamine/chalcone of 1.2:1 after 3h under reaction temperature of 65℃. The effects of reaction conditions on yields as well as the mechanism were studied and the yield was improved to 86.5% at the optimal conditions. Furthermore, the catalytic activity of CaO was greatly enhanced by surface modifying over CaO, and the effect of loading of modifier was also investigated. Besides, the protective effect on the apoptosis of PC12 cells induced by H2O2 by free radical scavenging of these compounds against H2O2 were evaluated by cell survival assay in PC12 cells using a reported methods.
Relationship Between Self-perception Burden and Sleep in Patients with Chronic Heart Failure in China
Chronic heart failure is a progressive disease, which is widely prevalent worldwide. The prevalence and mortality of chronic heart failure are increasing year after year. It has be bringing a heavy economic burden[1], which can make the patient have the thought of that they are the burden of others.This is the of the burden of self feeling ,which we named SPB for short. The existence of SPB could cause depression, anxiety, guilt, self remorse and other negative emotions[2]. The patients frequently suffer from physical and mental symptoms such as anxiety and dyspnea, which seriously affect the quality of sleep and lead to sleep disorders. Studies have find that sleep disorder is one of the main symptoms of chronic heart failure, the incidence of which is as high as 70%[3], and the quality of sleep will affect the prognosis of patients with [4-5]. However, SPB and sleep status in CHF patients have notbeen reported. In this paper, we take CHF patients as the object of study, and explore the relationship between SPB and sleep quality.
The Design and Practice of Process Guidance Method in Basic Nursing Standardization Skills Training
Objective: to investigate the effect of the process guidance method in basic nursing teaching of clinical nursing students. Method: to apply the process guidance method into clinical teaching to solve problems nursing students will encounter in their practice; to analyze the effect of teaching by questionnaires, nursing students operating completion rates and comprehensive evaluation results. Results: The operation (standardization) completing rates and comprehensive evaluation of the results from the experimental group are better than the control group. And the differences before using the process guidance method and after using it are statistically significant (P<0.05). Conclusion: The process guidance method enhances the effect of internship. It is a new clinical teaching mode of clinical applicability, which is worth being promoted.
Action Video Games Influence on Audiovisual Integration in Visual Selective Attention Condition
Visual and auditory stimuli filled every hole and corner of our lives. Audiovisual (AV) integration is an important cognitive capability involved in individual information processing. Previous researches show that the experience of playing action video games (AVG) will influence cognitive function. However, it is still unknown that whether the experience will affect AV integration processing function. We compared the AV integration processing function differences between Game Players and Non Game Players in visual selective attention condition. The results show Game Players’ level of AV integration function is lower than Non Game Players’, which means the experience of playing AVG will affect our audiovisual integration processing function.
Research Progress on Capacity Management in Patients with Chronic Heart Failure
Fluid retention is the first inducement in patients with decompensated heart failure (62.7%) and deterioration of heart failure[2]. The main reason for fluid retention is poor medication adherence and inability to strictly control the intake of fluids in the diet. It has not adjusted to diuretics according to their own conditions, such as high salt diet. Studies have shown that CHF patients' lack of self-management ability is to monitor body weight and urine daily and control liquid intake[3]. Xiaochun[4] investigated 178 cases of CHF, and found that 84.8% of the patients did not monitor the intake and output, 80.3% thought it was worthless to monitor the weight and intake, 66.3% did not know how to deal with the data of body weight and intake and output, 46.1% did not know the attentions of taking diuretics. The patient's capacity management is also affected by factors such as age, low cultural level, memory impairment, and visual loss. The inability of the medical staff to accurately assess and inform the patient's adjustment of liquid intake is also an important factor in capacity management. In the hospital, the best assessment of fluid state and monitoring of hemodynamics are important parts of nursing work, effective volume management strategy can effectively reduce the 30 day rehospitalization rate of patients with heart failure[5]. Improving the patient's ability to monitor body weight and access capacity and informing the patient's criteria for adjusting the amount of entry and exit are beneficial to CHF patients to identify symptoms early and avoid liquid retention.
Studies and Legislation on Euthanasia in China
In China, there is no legislation on euthanasia, but this does not mean that there is no problem of euthanasia in Chinese society. On the contrary, there are often reports of euthanasia, even criminal cases, from various media. However, it is necessary to explain that the lagging of the legislation of euthanasia is not the inaction of the legislator, but the complex character of the problem, because it involves the determination of the concept, the puzzle of ethics, the complexity of the legislation, and the diversity of concrete events in reality. Through combing the above problems and reviewing the previous legislative practice, I hope that some ideas and help can be provided to some extent for the future legislation and practice.
Optimization of Situ Thermosensitive Hydrogel System for Intranasal Delivery
Based on the physiological temperature of nasal cavity, the final hydrogel formulations and preparation method were determined. The screening matrix of thermosensitive hydrogel includes single factor, orthogonal design method and rheological properties. The results showed that the most suitable formulas were 19% P407 + 1.5% P188 + 8% HP-β-CD + 1.5% GCS and 19% P407 + 2% P188 + 8% HP-β-CD + 1.5% GCS. This situ thermosensitive hydrogel system can be used for intranasal delivery to treat CNS diseases.
Simultaneous Evaluation of Inhibition on Human CYP450 of Asarum Volatile Oil by a Cocktail Approach
In this study, the cocktail method was used to study the inhibitory effect of the volatile oil components including asarinin, methyl eugenol, asarone, safrole and myristicin on the CYP450 enzymes. Five kinds of probe substrates metabolized by different CYP450 enzymes were administered simultaneously in the system of co-incubation of the drug with human liver microsomes. The effect of the drug on the metabolism of the probe substrate was determined by the change of the metabolites of the probe substrate using UPLC/MS, and the inhibition of the enzyme was determined. The results showed that four drugs have an inhibitory effect on the metabolism of CYP450 enzymes except methyl eugenol, and myristicin has the strongest inhibitory effect on CYP1A2.
Docking Studies on a Series of Polo-like Kinase 4 Inhibitors
Polo-like kinase 4 (PLK4), a mitotic kinase was regarded as a potential target for cancer therapy. a novel series of PLK4 competitive kinase inhibitors have been docked into the active site of Polo-like kinase 4 (PDB code 4JXF) by using CCDC GOLD, furthermore, the binding modes for the inhibitors generated by MOE2015 may explain the mechanism of kinase selectivity.
Protein-based Alignment in 3D-QSAR of Polo-Like Kinase 4 Inhibitors
Polo-like kinase 4 (PLK4), a mitotic kinase was regarded as a potential target for cancer therapy. Three-dimensional quantitative structure–activity relationship (3D QSAR) studies were performed on PLK4 inhibitors, based on molecular docking obtained by using GOLD and comparative molecular field analysis (CoMFA). The high leave one out (LOO) cross-validated correlation coefficient (q2 = 0.531) reveals that the model is a useful tool for the prediction of test set as well as newly designed structures against PLK4 activity. The superimposed CoMFA models on the receptor site of PLK4 are guiding the design of potential inhibitory structures directed against PLK4 activity.
The Effects of Movement Methods on Patients with Idiopathic Facial Paralysis
This study is mainly the use of exercise therapy for idiopathic facial paralysis, while observing the clinical efficacy of patients, exercise therapy for the treatment of idiopathic facial paralysis to find evidence-based medical evidence. This is a randomized controlled trial, patients were divided into two groups, experimental group and control group, 20 cases in each group, the control group was given routine treatment, the experimental group on the basis of conventional therapy with exercise therapy, intervention on patients, namely brow lift closed training, training, training in training with the nose, teeth, lips training, training and other drum gills. The daily training 2 times, each training 30 times, for 1 month, one month after the two groups of patients using TFGS classification method to evaluate the quality of the results by t test, to observe the curative effect of two groups of patients, the exercise therapy in the treatment of facial neuritis is better than conventional therapy combined with conventional therapy, significantly accelerated the recovery the function of facial nerve.
Analysis of Causes of Mastitis and Assessment of Antibiotic Resistance Patterns in China
Mastitis in China is a common condition in breastfeeding as well as non-breastfeeding women. The causes of mastitis include congenital dysplasia, pathogen, and other diseases such as diabetes. The present study analyzed the main cause of mastitis and evaluated the antibiotic resistance patterns in cases with pathogen tested as positive. A total of 366 women were recruited in this study between October 2011 and May 2014. The abscess samples were obtained from the cases of mastitis with a breast abscess. The bacteria from these samples were isolated and cultured and isolated. Finally, the drug sensitivity of these cases was assessed by antibacterial susceptibility testing (AST). The congenital nipple dysplasia comprised of 52.9% (126/238) breastfeeding cases, while the non-breastfeeding bacterial infection comprised of 40.6% (52/128). The bacterial susceptibility test showed that the pathogen in both breastfeeding and non-breastfeeding cases showed extensive resistant to clindamycin, erythromycin, and penicillin. The rate of drug resistance in bacteria was lower in breastfeeding cases as compared to that in non-breastfeeding cases (P<0.05). The results showed that for the breastfeeding mastitis, the main cause is congenital nipple dysplasia, and bacterial infection in non-breastfeeding cases. For breastfeeding cases, emptying the breast and using ciprofloxacin, levofloxacin, and gentamicin as empirical choices before obtaining bacterial susceptibility test results is recommended. For non-breastfeeding cases, Oxacillin and Moxifloxacin could be used as empirical treatment.
Characteristics and Analysis of External Treatment of Vitiligo in Traditional Chinese Medicine
Objective: To explore the characteristics and advantages of external treatment of vitiligo by Chinese medicine. Methods: By studying the etiology and pathogenesis of the pathogenesis of vitiligo and comparing the current status of the treatment of the disease with traditional Chinese medicine, the characteristics of vitiligo treated with traditional Chinese medicine were analyzed. Results: It was clinically proven that the external use of Chinese medicine for the treatment of vitiligo is relatively effective compared to Western medicine treatment and internal medicine for the treatment of this disease. It can enhance the curative effect when used externally. Conclusion: The external treatment method has the characteristics of specimens, enhanced efficacy, direct access to the disease, simple and easy, and stable curative effect. The external preparations of traditional Chinese medicine have fewer side effects and have remarkable effects in the treatment of vitiligo. Vitiligo is a common skin disease caused by depigmentation of the skin. The symptoms are skin paleness and pigmentation, and the boundaries between normal skin and lesions are clear [1]. The incidence of vitiligo is more common, but the higher the skin color is, the higher the incidence of the race is, and the younger people have a higher incidence than the middle-aged and older people. In the later stages of the development of vitiligo disease, many serious diseases will be merged. The occurrence of this disease is easy to diagnose, but it is relatively difficult in the treatment process [2]. This article elaborates on the etiology and pathogenesis of vitiligo, the current status of treatment of traditional Chinese and Western medicine, and the characteristics of external treatment of traditional Chinese medicine, and hopes to provide some help for clinical treatment of vitiligo.
Clinical Efficacy of Kang’ai Injection Combined with Chemotherapy in Treating Advanced Cervical Cancer
Objective: To investigate the curative effect of Kangai injection combined with chemotherapy on advanced cervical cancer. Methods: 58 cases of advanced cervical cancer were randomly divided into two groups, observation group and control group, with 29 cases in each group. The control group was treated by conventional radiotherapy and chemotherapy. The observation group was given intravenous Kang’ai injection on the basis of radiotherapy and chemotherapy, and the treatment time was last for 4 weeks. According to the solid tumor evaluation criteria after the end of treatment to evaluate the short-term efficacy of patients, according to the Karnofskys performance scoring of patients with quality of life, and compared the two groups of patients treated by side effects. Results the effective rate of the observation group was 75.9%, while that of the control group was 44.8%. There was significant difference between the two groups (P <0.05), and the quality of life in the observation group was significantly better than that of the control group (P <0.01). The incidence of nausea and vomiting, leukopenia and abdominal pain in the observation group were significantly lower than those in the control group (P <0.05).Conclusions Kang’ai injection combined with chemotherapy in the treatment of advanced cervical cancer has obvious effect and little side-effect, which is a better choice for the treatment of advanced cervical cancer. It is worthy of clinical promotion.
Prognostic Value and Changes of Serum Connective Tissue Growth Factor Before and After Operation in Ovarian Cancer Patients
Objective: To explore the significance of changes of serum connective tissue growth factor (CTGF) level before and after operation in ovarian cancer patients. Methods The level of serum CTGF was detected in primary ovarian cancer patients pre- and postoperation (n=60, Group A), normal healthy subjects (n=60, Group B) by ELISA and analyzed the relationship between changes of serum CTGF before and after operation and clinical pathological features of ovarian cancer. Results The levels of serum CTGF in ovarian cancer patients before operation were significantly higher than patients with benign ovarian diseases and healthy subjects (all P<0.01), which declined significantly 1w after operation (P<0.01). The levels of serum CTGF before operation showed significant correlation with tumor size, TNM stages and lymph node metastasis (all P<0.01). Kaplan-Meier analysis showed that patients with high levels of serum CTGF had shorter survival than patients with low levels of serum CTGF (χ2=5.385, P=0.020). COX regression models showed that TNM stages, lymph node metastasis and the levels of serum CTGF (≥7.65ng/mL) were independent risk factors of influencing the survival time. Conclusion Serum CTGF in ovarian cancer was higher, which closely related to invasion and metastasis of ovarian cancer. It is valuable to detect the levels of serum CTGF for judging the prognosis of patients with ovarian cancer.
Retrospective Analysis of Risk Factors for Length of Stay in Intensive Care Unit and Survival Outcome from Premature Infants with Premature Rupture of Membranes
Objective: To analysis of risk factors for length of stay in intensive care unit and survival outcome from premature infants with premature rupture of membranes (PROM). Methods: A retrospective study including 189 singleton pregnant women with PPROM at 28– 34 gestational weeks was conducted. Gestational age at delivery, route of delivery, oligohydramnios, maternal age and maternal complications including gestational diabetes, preeclampsia and chorioamnionitis, newborn birth weight, gender, premature retinopathy (ROP), necrotizing enterocolitis (NEC), sepsis, fetal growth retardation (FGR), intracranial hemorrhage (ICH), bronchopulmonary dysplasia (BPD), respiratory distress syndrome (RDS), primary pulmonary hypertension (PPH), congenital cardiac disease (CCD), patent ductus arteriosus (PDA) and use of betamethasone were used to predict neonatal outcomes in terms of length of stay in the ICU and survival. Results: The preeclampsia, low birth weight, ROP, CCD, BPD, and PDA were significant confounders for length of stay in the ICU. Among them, low birth weight was the most powerful confounder for prolongation of the ICU stay (P<0.001). In multivariate logistic regression analyses, oligohydramnios (AFIâ‰¤ 5 cm), low birth weight, PDA, ROP were significantly correlated with neonatal survival. Low birth weight was the most powerful confounder in neonatal survival (P=0.001). Conclusion: Premature deliveries should be avoided to prevent infection and to prolong the latent period in cases of PROM in order to decrease prematurity related outcomes.
Analysis of Medicinal Value of Paulownia Flower
Paulownia flower contains flavonoids, triterpenoids, volatile oils, amino acids, proteins, tannins, phenols, trace elements and has the effect of detoxification, detumescence, anti-tumor, anti-oxidation, antibacterial and enhancing immunity. It has a good therapeutic effect on upper respiratory tract infection, bacillary dysentery, bronchial pneumonia, acute tonsillitis and conjunctivitis, otitis externa. The Paulownia flower is rich in resources, low price, but the market on Paulownia flower products rarely, lead to the Paulownia flower should not be fully applied, resulting in a waste of resources. For the comprehensive utilization of Paulownia flower, the flower of Paulownia chemical composition, pharmacological effects, clinical application, literature research into Line review.
Effect of Total Flavonoids of Herba Lysimachiae on Hyperuricemia in Mice Model
Objective: To observe the effects of total flavonoids of Herba Lysimachiae yeast extract with potassium oxonate hyperuricemia model mice. To observe the effects of total flavonoids of Herba Lysimachiae on model of hyperuricemia in mice induced by yeast extract combined with sylvite potassium salt. Method: In addition to the blank group, the mice in each group were treated with yeast paste for 13 days and intragastric administration for fourteenth days. After fourteenth days intragastric administration, by method for intraperitoneal injection of sylvite potassium salt replicated the mouse hyperuricemia model. After molding 8 days, large, middle, small dose group of total flavonoids of Herba Lysimachiae, Allopurinol Tablets group and Tongfengshu tablets group mice were fed with corresponding drugs at every afternoon, The blank group and the model group were given the same volume distilled water simultaneously Until 14d. Measured Serum uric acid (UA), urea nitrogen (BUN) and creatinine (CR) levels, serum xanthine oxidase (XOD) and adenosine deaminase (ADA) activity and observed the pathological condition of the kidney. Result: Total flavonoids of Herba Lysimachiae can inhibit the activity of ADA and XOD; decrease serum UA, CR, BUN level, improve the kidney disease. Conclusion: different dosages of Desmodium total flavonoids have a good improvement on the hyperuricemia model mice.
Effect of Raspberry on Osteoporosis Model in Ovariectomized Rats
Objective: To investigate the effects of raspberry on various indexes of serum and urine and bone mineral density in rats with osteoporosis. Methods: Female ovariectomized osteoporosis animal model was established by ovariectomy. Rats were completely removed the left ovary, the right removal of 80%, after careful breeding, intramuscular injection of penicillin 800000 u / kg (each 0.1ml) to prevent infection, continuous 3d, 1 day. After that, except for the model blank group, the rats in each group were given gavage for 60 days. The rats in the blank group were given the same amount of normal saline. The rats were fasted on the 58th day, Urine was taken 2h after the last administration on the 60th day. Blood was taken from the eye and the serum was separated. Serum calcium (Ca), phosphorus (P), serum BGP, urinary calcium, phosphorus, creatinine (Cr) and hydroxyproline (HYP) were measured. The tibias on one side of femur on both sides of the rats were used for bone mineral density, pathological examination and biomechanical measurement. Results: Compared with the blank group, serum Ca in the model group decreased significantly. High-dose raspberry group and Xianlinggubao group could significantly reduce the level of serum Ca, and middle-dose raspberry group also decreased serum Ca. Conclusion: The raspberry high, medium and low dose groups have a good intervention on osteoporosis model in ovariectomized rats.
Modern Research Progress of Jujube Leaves
Jujube is a unique tree species native to China. It has strong adaptability and can grow in drought, sand and alkali. It is cultivated throughout the country and is mainly produced in Henan, Hebei, Shandong, and Shaanxi. Jujube leaves are extremely rich in chemical composition and have significant medicinal value. Through consulting the relevant data in recent years, the chemical substances in jujube leaves have been summarized and analyzed, their pharmacological mechanisms have been deeply discussed, and the application of jujube leaves in medical and health care has been explored. , providing theoretical basis for the clinical application, new drug development and comprehensive utilization of jujube leaves. The jujube leaf is the leaf of Ziziphus jujube Mill. It was first introduced in the Shennongboshi Caojing. It was harvested in the spring and summer seasons. It was freshly used or sun dried. It was warm, sweet, and spleen, lung, and two classics. With heat dissipation and dehumidification. Jujube cultivation throughout the country, and large production. The study of jujube mostly tends to its fruit, jujube fruit has a high value of food and medicinal, has received widespread attention. In contrast, the study of jujube leaves is very rare. According to reports, jujube leaves are rich in flavonoids, triterpenoids and minerals, vitamins, proteins, carbohydrates, amino acids, etc., and their nutritional value and medicinal value are Very high. Jujube leaf as a rich potential resource has been buried by people for many years. According to statistics, the average annual output of jujube leaves per acre of jujube is 200kg. However, such rich natural resources are abandoned after the jujube has matured every year, resulting in A serious waste of resources.
The Effects of Huangqi Dihuang Pill on Immune Function of Immunosuppression Mice
Objective: Study the effects of Huangqi Dihuang pill(HDP) on immune function of immunosuppression mice. Methods: The mouse immunosuppression model was established by intraperitoneal injection of cyclophosphamide. intraperitoneal injection of chicken erythrocytes, intramuscular injection PHA, Then intraperitoneal injection of chicken erythrocytes and hennessy to detect the effects of Huangqi Dihuang pill on hemolysin, hemolytic plaque, lymphocyte transformation and phagocytosis of macrophages. Results: Huangqi Dihuang pill could improve the function of hemolysin, hemolytic plaque, lymphocyte transformation and macrophage phagocytosis of immunosuppression mice significantly (P<0.01). Conclusion: Huangqi Dihuang pill could improve the immune function of immunosuppression mice and is a potential immunomodulator.
Analysis of the Application Value of Peony Flower
Objective: To explore the medicinal and edible value of peony flower, and to make rational use of traditional Chinese medicine resources. Method: To understand the chemical composition, pharmacological action and edible value of the peony flower by consulting the literature and related information, and summarize and analyze, actively responding to the call of “Modernization of traditional Chinese medicineâ€. Result: The value of peony flowers is not only appreciated, but also has high medicinal and edible value. Therefore, the value of peony flowers should be further developed to lay a solid foundation for the development of traditional Chinese medicine and the rational use of medicines in clinical practice. Conclusion: Analysis of the application value of peony flower fully reflects the circular utilization of Chinese medicinal herbsâ€non medicinal partsâ€, and provides references for the development of Chinese medicine and the rational application of traditional Chinese medicine resources, so as to give full play to the application value of peony flower.
Observation on Current Situation of Higher Pharmaceutical Education in China from a New Perspective
Objective: This paper focuses on current higher pharmaceutical education in China with special emphasis on problems that exist. Those problems include: Rapid building up of programs based on demand for pharmaceutical specialists; Non-uniformity of program outcomes and ability to satisfy industry needs; Lack of clear educational goals and implementation means; General misunderstanding on how pharmaceutical education fits into existing higher education programs. Methods: On the basis of comprehensive consideration, a novel perspective, i.e., ecology, is adopted to integrate pharmaceutical education with existing medical education and to seek the solution to these problems. Results: Based on the analysis through ecological aspects, some measures are suggested that accurate orientation and characteristic development of higher pharmaceutical education should be carried out, including: diversification of higher pharmaceutical education, structure optimization of talent cultivation to meet social needs as well as cooperative innovation of colleges and enterprises. Conclusions: It is expected to exactly understand to the current situation of higher education in pharmaceutical speciality from ecologic perspective and provide a reasonable countermeasure.
The Application Progress of MicroRNA in Cerebral Ischemia-reperfusion Injury
Ischemia-reperfusion injury (I/R) is caused by the blood flow obstruction and is further aggravated by restoration of the blood supply suddenly. The brain is the most sensitive organ in the human body; ischemia can cause irreversible damage to brain cells after a few minutes. Therefore, exploring the prevention and treatment of cerebral ischemia-reperfusion injury are of great significance. In the current studies, scholars have found that microRNAs are closely related to the mechanism of cerebral ischemia-reperfusion injury, and gene therapy based on microRNAs is expected to be a new method for it.
Preparation and Application Possibility of Sodium Alginate/Chitosan Polyelectrolyte Membrane
As two excellent natural polymers, sodium alginate has excellent biocompatibility and chitosan has good chemical stability and fine bio-availability. They are eco-friendly and easily absorbed by human body. For this reason alginate and chitosan have been widely used in daily life and production, and some efforts were made to improve their application. In this paper a kind of polyelectrolyte membrane made from these two raw materials was prepared and its properties and possible application were studied. The results showed that the sodium alginate/chitosan (SA/CS) polyelectrolyte membrane is sensitive to pH and it hardly has swelling ability in acidic and alkaline solutions but swells easily in phosphate buffer saline (PBS) solution. Meanwhile, the SA/CS membrane has a high rate of water absorption and retention showing that it has a wonderful hydrophobicity and biocompatibility. The drug loading experiment showed than this SA/CS membrane has a smooth release rate of drug like ciprofloxacin hydrochloride indicating that it can be used as a drug carrier material and has a wide application prospect in biomedical material.
Modern Research and Application of Ginseng Flower
Ginseng is one of the most valuable medicinal materials in China, and has high application value. Ginseng roots, stems, leaves, buds and fruits have strong pharmacological activity and potential medicinal value. In modern research, the active and nutrient content of ginseng flower is not less than that contained in ginseng, and its application value is still to be developed. But at present, the research and application of ginseng flower is less. Through consulting the domestic literature in recent years, the main chemical components, pharmacological activities and related applications of Panax ginseng flower were summarized.
Correlation Between Serum Estradiol and Lung Cancer in Chinese Population
To analyze the correlation between the serum estradiol and lung cancer in Chinese population, PubMed, the Cochrane database and the China National Knowledge Internet database as well as VIP, WANGFANG MED, Sino Med were comprehensively searched for related studies strictly according to the selection criteria. Statistical analysis was performed using Review Manager 5.3 software. 16 studies were included for analysis, of which there were 736 subjects in the lung cancer group, 544 ones in the healthy control group. The results of Meta-analysis showed that the serum estradiol levels were higher in the lung cancer group than in the control one (SMD=0.81, 95%CI 0.31-1.30, Z=3.20, P<0.01). Meanwhile, from the subgroup analysis, the level of serum estradiol was higher in male lung cancer group (SMD=0.62, 95%CI 0.03-1.21, Z=2.06, P<0.05), but not in the female lung cancer group (SMD=0.02, 95%CI -0.51-0.55, Z=0.06, P>0.05) compared to the healthy control groups. Therefore, the serum estradiol level in the patients with lung cancer is higher than that in the healthy control population, suggesting serum estradiol level may be associated with the pathogenesis of lung cancer and expected to become a marker for the diagnosis of lung cancer, especially in Chinese population.
Application of Acute Hypervolemic Hemodilution Combined with Nitroglycerin Controlled Hypotension in the Elderly Patients
To explore the possibility of acute hypervolemic hemodilution combined with controlled hypotension with Nitroglycerin in the elderly patients. 30 elderly patients are randomly divided into three groups: pure application AHH group (Group I), AHH combined use of nitroglycerin group (Group II) and the control group (Group III). The HR, ECG, BP, SpO2, CVP, FIB, PT, APTT were determined in anesthesia (T0), cut skin before (T1), operation after the main steps (T2), and 24 hours after surgery (T3). Group I and Group III patients CVP value of the blood dilution (T1, T2) than baseline (T0) were significantly increased (P<0.05), Group II patients CVP value had no significant changes. Group I and Group II in the actual amount of bleeding significantly lower than Group III (P <0.05). Group II patients without intraoperative blood transfusion, significantly lower than that in Group I (P<0.05), significantly lower than that in Group III (P <0.01). Group I, Group II and Group III patients, the platelet count and fibrinogen significantly reduced (P <0.05), while PT, APTT was significantly longer (P <0.05), but were within the normal range. Acute hypervolemic hemodilution combined with Nitroglycerin controlled hypotension can enhance the effect of blood conservation, especially Nitroglycerin can lessen volume load and possess better myocardial preservation.
Direct Electron Transfer of Catalase and its Biosensing for H2O2
By immobilizing catalase on a functional nanocomposites containing ion-liquid, TiO2 nano particles and carboxylic acid functionalized multi-walled carbon nanotubes, direct electron transfer of CAT was realized on the nanocomposites modified glassy carbon electrode. The electrochemical study showed that the formal potential and heterogeneous electron transfer constant of the prepared modified glassy carbon electrode were −0.163 V and 5.24 sâˆ’1, respectively. The electrocatalysis study showed that the linear response range toward H2O2 and the apparent Michaelis–Menten constant were from 5 μM to 1000 μM and 54.3 μM, respectively.
Diagnosis and Treatment Analysis of Hilar Cholangiocarcinoma—Attached with an 18-case Report
Hilar cholangiocarcinoma (HCCA) refers to the malignant tumor occurring at left and right hepatic ducts, common hepatic duct and their convergence part. Its special anatomical position, adjoining relation and biological characteristics of infiltrating and metastasizing towards vessels, nerves and other surrounding tissues, its operative resection rate and long-term survival rate are extremely low so that HCCA diagnosis and treatment have become one of the difficult problems in the surgery department. This paper carried out a retrospective analysis of clinical data of 18 HCCA patients accepting operative treatment in our department from January, 2012 to December, 2016 to improve the recognition of HCCA diagnosis and treatment.
10% to 20% of patients who accepted cholecystectomy would have the symptoms such as recurrent upper abdomen or right upper quadrant pains, dyspepsia, or abdominal distension after the meal, or even infection of biliary tract and obstruction of biliary tract and so on several months, or years after the surgery[1,2]. The disease is clinically referred to as “postcholecystectomy syndromeâ€, and it refers to a group of diseases caused by a series of physiological and pathophysiological changes in human body after the gall bladder is excised. With the development of medical science and the deepened understanding of biliary tract diseases, some independent diseases that cause postcholecystectomy syndrome are identified and they are diagnosed and treated appropriately. Therefore, some scholars at home and abroad suggested that this ambiguous and vague diagnostic name should be abandoned; however, because the syndrome still contains a lot of diseases with unknown etiology, the term is still of clinical significance and it can’t be abandoned. However, this means that, when we treat this kind of patients, we need to thoroughly investigate his medical history, and try to find out the specific etiological factor through modern imageological examinations and provide reasonable treatments to the patients. Clinically, the postcholecystectomy syndrome can be divided into two parts of diseases, that is, those outside biliary system and those inside biliary system.
Report on one Case of Multiple Primary Carcinoma
Cases of double primary cancer (cholangiocarcinoma with gallbladder) are especially rare. We report a case which is special and more significant for future diagnosis and treatment compared with other cases. A female patient had main complaints of right upper quadrant pain for 1 month, and was diagnosed with gallbladder occupying, liver occupying, hepatic cyst, renal cyst and chronic cholecystitis with gallbladder stone after MRI examination. The patient was diagnosed with gallbladder cancer with liver and hepatic hilar lymph node metastases during the operation and a palliative operation was preceded. The tumors were diagnosed as double primary cancer (cholangiocarcinoma with gallbladder) based on postoperative pathology. A favorable treatment was given. Up to 10 month after surgery the patient died.
Experimental Study on the Effect of Shugan Jianghuo Granule on Hypertensive Rats with Hyperactivity of Liver Fire
Objective: The study based on previous studies that have confirmed spontaneously hypertensive rats (SHR) at 14-18 wk of age belong to stable phase of liver-fire hyperactivity and chose the SHR at age of 14-18 wk as animal model, preliminarily confirmed shuganjiaghuo granule’s hyperactivity effect by observed the effect of the drug on related microscopic physical and chemical indicators of rats with hypertension of liver-fire hyperactivity syndrome. Method: 32 male SHR at age of 15 wk belonged to liver-fire hyperactivity syndrome under the equivalent transformation of syndrome standard, randomly divided into shuganjianghuo granule high dose group, medium dose group and low dose group, model group, and 8 normal WKY rats as control group, collected animal macroscopic appearance, systolic pressure, rotating tolerance time, irritability level, after 4 weeks treatment, detected each group rats’ serum content of angiotensin II (Ang II) and norepinephrine (NE) by enzyme-linked immunosorbent (ELISE). Result: SHR at age of 14-18 wk was the stable phase of liver-fire hyperactivity syndrome. Shuganjianghuo granule had accuracy curative effect to treat hypertension and can improved the external manifestation of liver-fire hyperactivity, the medium dose group’s therapeutic effect was better than other groups. The theory of traditional Chinese medicine takes treatment based on syndrome differentiation as its core, and only the combination of disease and syndrome can fully explain the connotation of disease. Therefore, establish logical and stable animal models, as cornerstone and bridges, research microscopic physical and chemical indicators use advanced means and method could to provide objective criteria for diagnosis of syndrome and provide important reference standards for drug efficacy evaluation. According to previous studies and relevant literatures, spontaneous hypertensive rats (SHR) at the age of 14-18 wk showed liver-fire hyperactivity [1], and researches indicates that Ang II, NE significantly higher than other syndromes is important index [2] of hypertension of liver-fire hyperactivity syndrome. Shuganjianghuo Granule is used in hypertension of liver-fire hyperactivity syndrome widely by professor Zhou Lihua, gained a more satisfactory curative effect in clinical, has been granted the national patent.This study chose SHR aged 14-18 wk as the animal model, to observed the influence of shuganjianghuo granule different dose on microscopic index such as 419 macroscopic appearance, systolic pressure, rotation tolerance time, irritability level and Ang II, NE, preliminarily confirmed shuganjianghuo granule’s hypotensive effect and optimal dosage .
Experimental Study on the Effect of Shugan Jianghuo Granule on Related Indexes of Rats with Liver-fire Hypertension Syndrome
Objective: The study based on previous studies that have confirmed spontaneously hypertensive rats (SHR) at 14-18 wk of age belong to stable phase of liver-fire hyperactivity and chose the SHR at age of 14-18 wk as animal model, preliminarily confirmed shuganjiaghuo granule’s hyperactivity effect by observed the effect of the drug on related microscopic physical and chemical indicators of rats with hypertension of liver-fire hyperactivity syndrome. Method: 24 male SHR at age of 16 wk, randomly divided into model group, the Chinese medicine group (shuganjianghuo granule group), the Western medicine group (captopril group), and normal WKY rats as control group, collected animal macroscopic appearance, systolic pressure, rotating tolerance time, irritability level, after 3 weeks treatment, detected each group rats’ serum content of angiotensin II (Ang II) and norepinephrine (NE) by enzyme-linked immunosorbent (ELISE). Result: After 4 weeks treatment, compared with model group, shuganjianghuo granule group significantly lower systolic pressure and irritability, rotating tolerance time prolonged, serum Ang II, NE content reduced. And the captopril group the indexes were improved significantly except irritability degree. Conclusion: 16-18 wk of age SHR belongs to stable phase of liver-fire hyperactivity, shuganjianghuo granule has obvious steady and long hypotensive effect and can improved the manifestation of liver-fire hyperactivity. According to relevant literatures, spontaneous hypertensive rats (SHR) at the age of 14-18 wk showed liver-fire hyperactivity [1], and researches indicate that Ang II, NE significantly higher than other syndromes is important index [2] of hypertension of liver-fire hyperactivity syndrome. Therefore, this study chose WKY rats as the control group, as SHR aged 16 wk as the model group, to observed the influence of shuganjianghuo granule on microscopic index such as macroscopic appearance, systolic pressure, rotation tolerance time, irritability degree and Ang II, NE, preliminarily confirmed and appraised shuganjianghuo granule’s hypotensive effect.
Adsorption Performance of β-cyclodextrin and γ-cyclodextrin for Chlorpyrifos and Methomyl
The adsorption properties of β-cyclodextrin (β-CD) and γ-cyclodextrin (γ-CD) on two pesticides, Chlorpyrifos and Methomyl, were studied by ultraviolet visable spectroscopy. The experimental results showed that the molar adsorption efficiencies of equimolar β-CD on pesticides Chlorpyrifos and Methomyl were 0.8452 and 0.7373, while the adsorption efficiencies of equimolar γ-CD on pesticides Chlorpyrifos and Methomyl were 0.8514 and 0.7427, respectively. There were no sharp distinction between the adsorption efficiencies of β-CD and γ-CD on them. However, pesticide residues of chlorpyrifos and methomyl in food, fruits and vegetables could be effectively removed by β-CD or γ-CD. Considering the economic factors, β-CD is the preferred and convenient pesticide remover.
Usefulness of 18F-FDG PET/CT for Evaluating Response of Breast Cancer to Neoadjuvant Therapy
Neoadjuvant chemotherapy (NACT) was introduced more than four decades ago for the treatment of locally advanced breast cancer. Accurate assessment of the residual disease after NACT is important for further surgical management. The aim of this study was to document the appearance of 18F-FDG PET/CT after chemotherapy and correlate them with histopathology, to calculate the accuracy of 18F-FDG PET/CT in predicting the size of the residual disease. We undertook a retrospective review of patient data for 38 women who had histologically confirmed malignant tumor with at least 1 site and underwent FDG PET/CT before and after treatment between January 2016 and December 2017. Anti-treatment and post-treatment findings of both PET/CT, ultrasonic were collected and analyzed according to the pathological results. In our analysis of 38 patients treated with neoadjuvant therapy, after chemotherapy there was reduction in size of all of the measurable masses (38/38,100 % of mass lesions) and in lesion number of 23 cases. And there was a significant decrease of both diameter (36/38) and number (19/38) of positive lymph node. We took SUV max and SUV mean of lymph node as indicator, and we found that the SUV max showed a decline in 35 cases of breast and in 33 cases of positive lymph node, and the SUV mean showed a decline in 38 cases of breast and in 32 cases of positive lymph node. In conclusion, PET/CT was more accurate in evaluating tumor size than ultrasonic examination, besides, in T2 and T3 stages, SUV max and SUV mean could be an indicator for neoadjuvant therapy.
The Effect of Polyhexamethylene Biguanide Complex Irrigation in Polyacrylamide Hydrogel Removal Operation of Breast
Polyacrylamide Hydrogel (PAHG) has been commonly used as medical filling agent in the treatment of a variety of soft tissue defect disease. However, when PAHG breaks down into monomers in human body, it could cause a series of complications. In China, Polyacrylamide Hydrogel removal operations are necessary for a large number of patients who had received breast augmented operation with PAHG and were suffering with its complications. Polyhexamethylene biguanide (PHMB) complex has been proved to be effective in wound with inflammation. The aim of this study was to evaluate effect of Polyhexanide-betaine irrigation in Polyacrylamide Hydrogel removal operation of breast. We undertook a prospective study of 32 patients who underwent bilateral PAHG removal operation of breasts between June 2016 and December 2017. Intraoperative irrigation with Polyhexanide-betaine solution was taken in one side (PHMB group), and with saline in the other side (saline group). Indicators of recovery after the operation in two groups werecollected and used to evaluate the effect of PHMB. In our analysis of 32 patients treated with PHMB irrigation, the volume of drainage was significantly reduced in PHMB group compared with saline group (P<0.05). The period with the drainage tube has been obviously shortened in PHMB group compared with saline group (P<0.05). And The VAS score in the PHMB group was significantly decreased ompared with the saline group 48 hours after the operation. In conclusion, the Polyhexanide-betaine solution has a positive effect on Polyacrylamide Hydrogel removal operation of breast. It could help to reduce the total amount of drainage and shorten the recovery period.
18F-FDG PET/CT of Breast Cancer for Preoperative Evaluation of Sentinel Node Metastasis
Axillary lymph node (ALN) metastasis is one of the most important aspects for breast cancer patients’ prognosis; however ALN dissection it is associated with a series of long-term complications. Sentinel lymph node (SLN) biopsy was performed as another procedure to minimize the adverse impact of axillary surgery. Accordingly preoperative prediction of axillary lymph node metastasis (ALNM) by image examination or biopsy of SLN is of great significance to select patients with low risk of ALNM and avoid full axillary lymph node dissection (ALND). In this study, we evaluated preoperative evaluation of sentinel node metastasis by using 18F-FDG PET/CT to staging axillary lymph node status. We undertook a retrospective review of patient data for 275 women who had histologically confirmed breast carcinoma by needle biopsy and underwent 18F-FDG PET/CT examine between March 2015 and April 2018. ALND or SLN biopsy were performed on 268 cases and 18F-FDG PET/CT results were evaluated according to final pathological results. Of 268 patients, 137 cases (79.7%) showed ALNM by surgical histopathology analysis. And we observed that comparing with ALNM group the number of positive lymph node diagnosed by PET/CT in false positive group was significantly lower (P<0.05), while the max diameter of both breast lesion and positive lymph node diagnosed by PET/CT were longer (P<0.05).By using immunohistochemistry staining and classification of molecular subtype of breast cancer, we observed that compared with ALNM group, the ration of HER2 overexpressed breast cancer in false positive group was significantly higher (P<0.05). In conclusion, despite the low false positive rate 18F-FDG PET/CT for preoperative evaluation of sentinel node metastasis is of great value for surgeons to select patients with low risk of ALNM and avoid full ALND.
Pathological Analysis of Non-cancerous Lesion in Breast with Abnormal FDG Absorption in 18F-FDG PET/CT Test
18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) (18F-FDG-PET/CT) is a sensitive molecular imaging modality capable of diagnosing breast cancer by means of increased FDG uptake in growing cancer cells. FDG uptake within cells mainly depends on cellular metabolic activity and the number of glucose transporters. However, this mechanism is not specific to cancer cells, for FDG can accumulate in inflammatory cells and benign processes causing false-positive findings. In this study, we analyzed the non-cancerous lesion diagnosed by histology which showed abnormal absorption of FDG in 18F-FDG PET/CT test and demonstrated the image feature of these cases. We undertook a retrospective review of data from 373 women who had an 18F-FDG PET/CT test and analyzed the false positive results in 48 cases according to final pathological results between January 2016 and April 2018. Among these cases, 296 showed abnormal FDG absorption and were histological diagnosed after surgery. Of 296 patients, who had abnormal FDG absorption, 248 cases were histological diagnosed with breast carcinoma. Fibroadenoma and purulent inflammation accounted for 33.3% and 35.4% of all 48 non-cancerous cases following by intraductal papilloma with the incident of 12.5%. In purulent inflammation cases the max diameter of tumor showed significantly larger than others (P<0.05), and the max diameter of lesion in other diseases showed no significantly differences. 18F-FDG PET/CT results showed that the value of SUVmax in purulent inflammation was significantly higher than that in other diseases (P<0.05) and adenomyoepithelioma, cyst with ductal ectasia and adenosis were showed with a lower SUVmax (P<0.05). In conclusion, despite the low false positive rate 18F-FDG PET/CT is of great value for preoperative evaluation of breast lesion. In the non-cancerous cases which had an indication of carcinoma in 18F-FDG PET/CT test, the most common diagnosis were fibroadenoma, purulent inflammation and intraductal papilloma.
Temperature Characteristics and Compensation of PET Detector
The detector is the core component of PET. Temperature of the detector has a significant impact on the quality of PET image. The operating temperature of the machine can result in reduced spatial resolution, declining image quality and diagnostic errors. Faced with the issue, a set of temperature control platform was built and the experiment about the temperature characteristics and compensation of PET detector was completed. Through the analysis of peak drift caused by the temperature change and the exploration of the law about high voltage compensation, it is concluded that the temperature, the peak and the high voltage have a certain linear relationship. Finally, the compensatory method for peak drift is summarized.
The Fuzzy Pattern Recognition for Motor Imagery EEG
Motor Imagery Electroencephalography (MI-EEG) is a nonlinear signal with multiscale and fuzzy characteristics and its recognition has good applications for the rehabilitation of stroke or hemiplegia patients. In the previous study, few of experts develop fuzzy recognition methods from the point of MI-EEG signal characteristics. In this paper, the improved Multiscale Fuzzy Entropy (IMFE) is integrated with Fuzzy Support Vector Machine (FSVM) based on Fuzzy C-Means (FCM) to yield a novel fuzzy recognition method, denoted as IF-FSVM. IMFE is applied to extract the features of MI-EEG signals. And the fuzzy memberships of the features are calculated by using FCM. Furthermore, FSVM is employed as a classifier to recognize the pattern of movement imagination for MI-EEG. Experiments are performed on a public BCI competition dataset, and the results of 10-fold Cross Validation show that IF-FSVM yields relatively higher classification accuracy compared with some common used recognition methods.
Isosbestic Point of Traditional Chinese Medicine Injections (TCMIs) and Its Practice in Evaluating TCMIs’ Stability
The aim of the study was to discover the isosbestic point of Traditional Chinese Medicine Injections (TCMIs) and to verify its practice in evaluating TCMIs’ stability. Six TCMIs (Qingkailing, Shuanghuanglian, Danshen, Dengzhanxixin, Honghua, and Shuxuening Injection) were kept at 60 oC, and solutions of 1 ml were sampled when heated for 0, 1, 2, 3, 4, or 8 weeks. Samples were diluted and their ultraviolet-visible spectrum was scanned from 230-800 nm. Isosbestic point of the TCMI was picked out. AUCB (area under the spectrum curve before the isosbestic point) or AUCA (AUC after the point) was obtained, and the BA ratio was calculated by AUCB/AUCA. HPLC-fingerprints of the sample were performed and total area of fingers (TAF) was calculated. TCMIs’ color was darkened when heated for weeks. During heating, AUCB decreased and AUCA increased, while BA ratio was increasing. The value of BA ratio from all the heated TCMIs was ten percent smaller than that of the original one, so do the results of HPLC-fingerprints except Honghua Injection. There was a good relationship between BA ratio and TAF except Honghua Injection. All the results suggested that isosbestic point existed, and BA ratio was a sensitive index to evaluating TCMIs’ stability.
A Method for the Measurement of Second Heart Sound Split Based on Modified S Transform
The time delay between A2 and P2, which is known as S2 split is reported to have significant diagnostic potential. This paper proposes a target function to optimize the standard S transform, and the genetic algorithm (GA) is employed to select the optimal parameter, so that the modified S transform has a better measure ability for S2 split. In order to evaluate the performance of the proposed method, a quantitative experiment was performed on simulated S2 signals with different split duration between A2 and P2. The effectiveness was compared with the standard S transform and another modified version of S transform.
The Catalytic Performance of Penicillin G Acylase in Different Organic Solvents
This work investigated the catalytic performance of PGA engineered with a variety of functional groups in organic solvents at room temperature for different interval, such as nalkanes, chlorinated methane, alcohols and acids. The major research highlights are listed as: â‘ With the extension of time, as for hydrophobic organic solvent, catalytic activity retention rate (Cr) decreased and achieved equilibrium gradually, in a series of solvent, the smaller log P, the lower Cr; as for hydrophilic organic solvent, Cr increased first and then decreased to an equilibrium value, in a series of solvent, the smaller log P, the higher Cr. ②With the increasing of the solvent molecular size (l), equilibrium Cr achieved showed different trends: when l < 4.4 Ã…, equilibrium Cr achieved was fast and not affected by l; when 4.4 Ã… < l < 6.9 Ã…, it was slow and increased with the increasing of l, when l > 6.9 Ã…, it was fast and decreased with the increasing of l. â‘¢ In the series solvents, PGA treated with polyalcohol, especially glycerine, its Cr reached maximum and maintained stability.
Infection Regularity of Rhesus Cytomegalovirus in Indigenous RhCMV Rhesus Monkey Model
RhCMV, which is a counterpart of HCMV, some specific phenotypes of natural infection are not fully understood. The present research was designed to study the seroprevalence of indigenous infection of RhCMV among different age groups by ELISA, and detected its histological distribution in adult and their own fetal monkeys by the ISH and PCR methods. The ELISA result showed that the average seroprevalence of RhCMV was 76%, the concentration of RhCMV-IgG in each group was between 10- 30 pg/ml and there was no significant difference between each group (P>0.05). The ISH and PCR results showed that RhCMV infected a wide range of tissues, including immune system, respiratory tract, gastrointestinal tract and urinary tract, and the positive signal of RhCMV in mother monkeys was stronger than that of in their own fetal monkeys. In addition, we also found that RhCMV positive signal also existed in mother’s reproductive organs, including uterus, ovary and placenta. The above results indicate that RhCMV can infect all age groups monkeys and hide in a wide range of organs, and can transmit vertically to their own fetal. The infection regularity of indigenous RhCMV rhesus monkey model can provide support for further understanding the persistence and pathogenesis mechanism for HCMV. Furthermore, these results can remind the researchers to give more attention to the potential and adverse effect induced by RhCMV when doing reproductive work and some other research work.
Antioxidant Properties of Different Molecular Weight Polysaccharides from Seaweed Sargassum Fusiform
This study comparatively analyzed the antioxidant activities of original and three degraded sargassum fusiform polysaccharide fractions, named SFP0, SFP1, SFP2, and SFP3, with molecular weight of 16.2, 8.21, 3.83, and 2.23 kDa, respectively. Results revealed that the scavenging activity of various SFP fractions for hydroxyl, DPPH, and ABTS radicals and the reducing power increased with decreasing molecular weight of SFP within 16.2-2.23 kDa. SFP3, with the lowest molecular weight of about 2.23 kDa, exhibited the strongest radical scavenging capacity. Moreover, the Fe2+ chelating ability also increased with decreasing molecular weight of various SFP fractions. The active groups (such as -OSO3H) of low-molecular-weight polysaccharides was easily exposed and possessed high degree of freedom and low space steric hindrance; hence, their chelating ability increased. These SFP fractions, especially SFP3, may be potential anti-stone drugs.
Differences in Adsorption of Anionic, Cationic and Nonionic Surfactants on Calcium Oxalate Crystals: Effect of Crystal Size
The adsorption differences of surfactants with different types of charges onto nano/micron calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals were investigated to explore the effects of urinary molecules with different types of charges on formation of urinary stones. These surfactants include anionic surfactant sodium dodecyl sulfate (SDS), cationic surfactant cetyltrimethyl ammonium bromide (CTAB), and nonionic surfactant nonylphenol ethoxylate (NP-40). The sizes of COM and COD crystals are 100 nm and 10 μm, respectively. The adsorption capacity of different surfactants on nano/micron COM and COD crystals and the Zeta potential on crystal surface after adsorption were measured. The settling velocity of the crystal suspensions after adding different surfactants was compared. The adsorption quantity of three surfactants on COM and COD was ranked in the following order: SDS > CTAB > NP-40. The adsorption quantity of COM was greater than that of COD with the same size. It indicated that the adsorption capacity is related to the crystal structure of the crystal surface. The absolute value of Zeta potential on surface of COM and COD increased after adsorption of surfactants. The crystal settling velocity was negatively correlated with the amount of surfactants adsorbed and the Zeta potential of the crystals. Surfactants can interact with the calcium sites or oxalate sites on the crystal surface. There are different interactions between different surfactants and nano/micron crystals. We proposed a molecular model of crystal adsorption with different surfactants. Larger adsorption quantity of surfactants can slower the sedimentation rate of crystals and thus stabilizes crystal suspension, finally inhibits the formation of calcium oxalate stones.
Effects of Tea Polysaccharide on Crystallization of Calcium Oxalate
The effect of tea polysaccharide (TPS) on crystal growth of calcium oxalate (CaOx) was studied. X-ray diffraction and Fourier transform infrared spectroscopy(FT-IR) results revealed that TPS inhibited crystal growth, and increased the absolute value of zeta potential on crystal surface and thus favorably inhibiting crystal aggregation. In the concentrations of 0 to 3.0 g/L, TPS only induced calcium oxalate monohydrate (COM) formation, but the crystal face strength I( 101)/I(020) of COM increased. These results indicated that TPS may be a potential drug for the prevention and treatment of CaOx stones.
Depression Status and Influencing Factors Among the Rural Poor Elderly in Poverty-stricken Townships
Objective To describe the depression status of the rural poor elderly in poverty-stricken townships, and analyze the factors that influenced the depression status. Methods A cross-sectional survey was conducted with a structured questionnaire among 249 old people at the age of 60 or older in August 2013 in eight villages of Zhuchangping township in Xingyi city, Qianxinan autonomous prefecture of Buyei and Miao minorities, Guizhou province. The depression was measured by the geriatric depression scale (short form), and multivariate logistic regression method was applied to analyze influencing factors. Results The component ratio of depression among the poor elderly was 81.6% (a mild depression of 23.7%, a moderate depression of 26.3%, and a severe depression of 31.6%), higher than among the non-poor elderly with a statistically significant difference (P=0.01). Compared with male, non-poor and not walking difficultly elderly people, female, poor and walking difficultly elderly people were more likely to report the depression with the OR value of 3.10, 4.16, and 2.08, respectively. Compared with the elderly who had not received objective supports, subjective supports within family, and not met with migrant children in one year, the elderly who had received objective supports, subjective supports within family, and met with migrant children in one year were less likely to report depression with the OR value of 0.39,0.52,and 0.32, respectively. Conclusion: The status of depression was bad among the rural poor elderly in the poverty-stricken township. The influencing factors were gender, difficulty in walking, objective support, subjective support within family, and meeting with migrant children in one year.
Detection of EPA and DHA in the Viscera of Marine Organisms
The viscera of 6 kinds of non-fish marine organisms were collected for detection of DHA/EPA using gas chromatography. The oil extraction ratio varied from 0.72% to 6.23%(wt%). Scylla serrata presented the highest oil yield, followed by Architeuthis dux and Panopea abrupta. The EPA concentration (in methyl ester form) in the oil varied from 0.56 to 7.75(mg/g). Scylla serrata presented the highest EPA concentration(p<0.05), followed by Architeuthis dux and Panopea abrupta. The DHA concentration(in methyl ester form) varied from 0.15 to 4.71(mg/g). Architeuthis dux and Scylla serrata presented higher DHA concentration than other species(p<0.05).
In Vitro Neurotoxicity Evaluation of Betel Nut Decoction on the Neurons/Astrocytes Co-cultured Cells
The betel nut has attracted great research interest for their potential applications inpharmacy, food and biomedical areas. However, little information of their neurotoxicity isreported. Herein, we performed a comprehensive study on the neurotoxicity of betel nutdecoction (BND) by examining the influences of betel nut decoction on the morphology,viability, LDH release, intracellular Ca2+ concentration, mitochondrial membrane potential,synaptic plasticity-related proteins (Arc, SYN, β-III-Tubulin), neurotransmitters and glutamateof co-cultured cells. The results showed that the betel nut decoction had general toxic effects onco-cultured cells. The general toxicity indexes showed that 150 μg/mL group increased LDHrelease, increased intracellular calcium concentration and decreased mitochondrial membranepotential. Moreover, 50 μg/mL betel nut decoction treated with neurons/astrocytes co-culturedcells for 6 h caused neurotoxicity, SYN can be used as a sensitive protein for rapid detectionbetel nut decoction neurotoxicity. These results are dose related, and should be considered inthe development of bio-applications of betel nut, which provides the basis for future studies onthe mechanism of drugs-induced neurotoxicity.