Numerical Simulation of Immunoisolated Co-culture Bioreactor for in Vitro Expansion of Human Hematopoietic Stem Cells

Abstract

Bioreactors are good devices for biological and chemical reactions. In this paper, two-dimensional flow fields and three-dimensional flow fields were simulated using Gambit and Fluent software to determine the shear stress and velocity curves of the immunoisolated co-culture bio-reactor system (ICBS). For different speeds and S-curvature curvature, the mass transfer effect and flow field of the two sides are calculated to find suitable conditions for cell growth. It was found that the growth of cord blood CD34+ cells was most favorable when the 10 rpm co-rotation condition in the case of a two-dimensional flow field. The three-dimensional flow field focuses on the shear force of the double-tube side fluid. It is found that the shear force increases first and then decreases in the near inner wall surface area; the shear force increases gradually on the near outer wall surface.