Exposure of Zebra Fish (Danio rerio) to Different Concentration of Cadmium

Abstract

Cadmium is a natural element found in the earth’s crust. It is usually combined with other metals such as calcium or chromium. In single and associated form this metal is highly toxic. Our study is based on exposure of a fresh water zebra fish species named Danio rerio. This species is commonly used for metal exposure studies. Research was done by exposing the zebra fish to different concentration of cadmium (5 μg/L, 10 μg/L and 15 μg/L) for 8 days. Sampling was done on 0, 2, 4, 6, and 8 day respectively. Samples were frozen at -80 degrees. Physical parameters such as temperature, pH and salinity was checked and maintained regularly Later 1g of each sample were taken and digested through nitric acid digestion method. Concentration of cadmium accumulated in body of zebra fish was determined through the Atomic Absorption Spectrophotometer. Result showed the concentration of cadmium in body and number of deaths increased with increased in duration of exposure.