Study on Isolation of a Phosphate Removal Organism and Influencing Factors of Phosphate Removal Efficiency

Abstract

In order to obtain high efficiency phosphate removal strains. Some pure strains were isolated from the activated sludge from biological phosphorus removal(BPR) reactor after the aerobic phase end. After staining of the metachromatic granules and phosphate removal test, strain P10 with high phosphate removal efficiency was isolated. By homology comparison of 16SrRNA gene and physiological and biochemical identification, the strain P10 is identified as Pseudomonas sp. and registration number in GenBank is KX181643. Contrast experiment under different substrate, temperature and pH showed, strain P10 is able to make good use of acetate and propionate to remove phosphorate while the poor utilization of glucose. Optimum phosphorus removal temperature range of strain P10 is15-30oC and pH range is 6-7.The strain P10 has high phosphate removal ability after comparison.