The Biological Activation of Fairy Shrimp Cyst Induced by Ultrasound Exposure

Abstract

Fairy shrimp is an anostraca plankton which is raised for watching and scientific study. This thesis is then focusing on the biological activities to increase the hatching rate of fairy shrimp by light and ultrasound exposure. This work investigates the light effect on the hatching experiment using different wavelength and intensity of LED light; in addition, Cysts is exposed to ultrasound by ultrasonic cleaner and transducer. The resonant frequency of the Cysts is obtained from Rayleig–Plesset bubble activation formula. The radiation of the resonant and non-resonant regions during the hatching experiments are then set up by this resonant frequency for comparison. Finally, according to the experimental results, the correlations of hatching rate with light wavelength, light intensity, resonant frequency and ultrasound intensity are studied by Taguchi Method to understand the play role of the light and ultrasound. The research results show that the blue light plus ultrasound frequency of 0.25 MHz plus ultrasound intensity of 30.9 mW/cm2 can let the incubate percentage up to 48.3%. This is the highest incubate percentage one can get from these experiments. The results of this research can be referred by an endangered species or high economic value of species to increase the rate of hitching.