Simultaneous Determination of Sulfonamide, Quinolones, Tetracycline and Macrolide Antibiotics in Soils Using ASE-SPE-HPLC/MS

Abstract

This research developed the methods for Simultaneous determination of 4 kinds of antibiotics in soils. Acetonitrile-phosphate was used as extraction solvent, Diatomite(washed by EDTA) was used as dispersing agent. Firstly, soil was extracted by ASE with the parameter conditions: pressure 1500 psi, temperature 70 ℃ , static 10 min, 1 circle, then pre-concentration by SPE, followed by HPLC-MS/MS analysis. Recovery is 72.3% ～ 97.6% for SAs, 63.2% ～ 93.2% for FQs, 60.2% ～ 82.3% for TCs and 67.2% ～ 78.9% for MLs. RSD0.99. Limits of detection is 0.5 ～ 0.9μg /kg for SAs, 1.3 ～ 1.7 μg/kg for FQs, 0.2 ～ 1.1μg /kg for TCs and 0.2 ～ 0.3μg /kg for MLs. This method detects 15 kinds of antibiotics in 15 min, the detection accuracy can truly meet the requirements. The Diatomite(washed by EDTA) can increase the recovery of most of the antibiotics. Therefore, this method is worth learning.