Preparation of Magnetic Fluorescent Core-shell-structured Nanoparticles and Their Application in Immobilization of Glucose Oxidase

Abstract

The magnetic fluorescent core-shell structured nanoparticles, Fe3O4 @SiO2 (F) @meso-SiO2 nanoparticles (FSMSNp), with average diameter of 240 nm have been prepared and characterized. Glucose oxidase (GOD) was immobilized on them to form immobilized GOD. After immobilization, the storage stability of GOD was improved. Since the fluorescence of complex nanoparticles can be quenched by oxygen, these nanoparticles could have the potential application in multi parameter fiber optic biosensor based on enzyme catalysis and oxygen consumption.